Haolu Wang, Camilla A. Thorling, Zhi Ping Xu, Darrell H. G. Crawford, Xiaowen Liang, Xin Liu, Michael S. Roberts
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引用次数: 3
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Abstract
This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs are described. Protocols for data analysis are suggested, with the goal of developing a model to characterize and predict the physiological kinetics of administered MSCs. The use of this model is described, suggesting that it can be applied to abnormal conditions and has potential interspecies and inter-route predictive capability. These universal methods can be employed, regardless of the type of stem cell or disease, to guide future experiments and design treatment protocols. © 2017 by John Wiley & Sons, Inc.
间充质干细胞体内分布的可视化和建模
本单元描述了一个方案，阐明体内处置的管理间充质干细胞(MSCs)。具体来说，描述了直接可视化供体细胞的时空分布和评估供体细胞在受体器官中的数量。提出了数据分析的方案，目的是建立一个模型来表征和预测给药MSCs的生理动力学。结果表明，该模型可以应用于异常条件，具有潜在的种间和路线间预测能力。无论干细胞或疾病的类型如何，这些通用方法都可以用于指导未来的实验和设计治疗方案。©2017 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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