Roberta Pecoraro, Antonio Salvaggio, Fabio Marino, Gianfranco Di Caro, Fabiano Capparucci, Bianca Maria Lombardo, Giuseppina Messina, Elena Maria Scalisi, Maurizio Tummino, Francesco Loreto, Giusi D'Amante, Roberto Avola, Daniele Tibullo, Maria Violetta Brundo
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引用次数: 29
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Abstract
Nanomaterials (NM) have different shapes and can be composed of different materials such as carbon, silicon, and some metals like gold, silver, and titanium. They are used as fillers, catalysts, semiconductors, cosmetics, drug carriers in medicine, energy storage systems, and antifriction coatings. NM are the parent compounds of nanoparticles (NPs), which may be divided into two groups: fullerenes and engineered nanoparticles (ENPs). After crossing the cell membrane, NPs may be stored in vesicles, mitochondria, and additional organelles within epithelial cells. They may generate reactive oxygen species, oxidative stress, and cytotoxicity, as well as denaturation of proteins, apoptosis, and necrosis. Nowadays, new toxicological data are required to assess the potential exposure of the environment and human beings to pollutants. The aim of the present investigation is to evaluate the toxicity of the metallic nano-composite by the zebrafish embryo toxicity test (ZFET). The methods described here can be easily adapted to other nanocomposites or nanomaterials with some variations. © 2017 by John Wiley & Sons, Inc.
利用斑马鱼胚胎毒性试验评价金属纳米复合材料的毒性及特异性暴露生物标志物的鉴定
纳米材料(NM)具有不同的形状,可以由不同的材料组成,如碳、硅和一些金属,如金、银和钛。它们被用作填料、催化剂、半导体、化妆品、医药中的药物载体、储能系统和防摩擦涂层。纳米颗粒是纳米颗粒(NPs)的母体化合物,可分为两类:富勒烯和工程纳米颗粒(ENPs)。穿过细胞膜后,NPs可能储存在上皮细胞内的囊泡、线粒体和其他细胞器中。它们可能产生活性氧、氧化应激和细胞毒性,以及蛋白质变性、细胞凋亡和坏死。目前,需要新的毒理学数据来评估环境和人类对污染物的潜在暴露。采用斑马鱼胚胎毒性试验(ZFET)评价金属纳米复合材料的毒性。本文描述的方法可以很容易地适用于其他纳米复合材料或具有一些变化的纳米材料。©2017 by John Wiley &儿子,Inc。
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