Pamela J. Roqué, Lucio G. Costa
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引用次数: 30
Abstract
Microglia, the resident immune cells of the brain, have been implicated in numerous neurodegenerative and neurodevelopmental diseases. Activation of microglia by a variety of stimuli induces the release of factors, including pro- and anti-inflammatory cytokines and reactive oxygen species, that contribute to modulating neuro-inflammation and oxidative stress, two crucial processes linked to disorders of the central nervous system. The in vitro techniques described here will provide a set of protocols for the isolation and plating of primary cerebellar granule neurons, primary cortical microglia from a mixed glia culture, and methods for co-culturing both cell types. These methods allow the study of how microglia and the factors they release in this shared environment mediate the effects of toxicants on neuronal function and survival. The protocols presented here allow for flexibility in experimental design, the study of numerous toxicological endpoints, and the opportunity to explore neuroprotective strategies. © 2017 by John Wiley & Sons, Inc.
神经元与小胶质细胞的共培养。
小胶质细胞是大脑的常驻免疫细胞,与许多神经退行性和神经发育性疾病有关。通过各种刺激激活小胶质细胞,诱导因子的释放,包括促炎性和抗炎性细胞因子和活性氧,这些因子有助于调节神经炎症和氧化应激,这两个关键过程与中枢神经系统疾病有关。这里描述的体外技术将提供一套分离和镀初级小脑颗粒神经元的方案,混合胶质细胞培养的初级皮层小胶质细胞,以及两种细胞类型共培养的方法。这些方法允许研究小胶质细胞及其在共享环境中释放的因子如何介导毒物对神经元功能和存活的影响。这里提出的方案允许实验设计的灵活性,许多毒理学终点的研究,并有机会探索神经保护策略。©2017 by John Wiley & Sons, Inc。
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