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{"title":"Finding Homologs in Amino Acid Sequences Using Network BLAST Searches","authors":"Istvan Ladunga","doi":"10.1002/cpbi.34","DOIUrl":null,"url":null,"abstract":"<p>BLAST, the Basic Local Alignment Search Tool, is used more frequently than any other biosequence database search program. We show how to run searches on the Web, and demonstrate how to increase performance by fine-tuning arguments for a specific research project. We offer guidance for interpreting results, statistical significance and biological relevance issues, and suggest complementary analyses. This unit covers both protein-to-protein (<i>blastp</i>) searches and translated searches (<i>blastx, tblastn, tfastx)</i>. <i>blastx</i> conceptually translates the query sequence and <i>tblastn</i> translates all nucleotide sequences in a database, while <i>tblastx</i> translates both the query and the database sequences into amino acid sequences. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":10958,"journal":{"name":"Current protocols in bioinformatics","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpbi.34","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpbi.34","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
BLAST, the Basic Local Alignment Search Tool, is used more frequently than any other biosequence database search program. We show how to run searches on the Web, and demonstrate how to increase performance by fine-tuning arguments for a specific research project. We offer guidance for interpreting results, statistical significance and biological relevance issues, and suggest complementary analyses. This unit covers both protein-to-protein (blastp ) searches and translated searches (blastx, tblastn, tfastx) . blastx conceptually translates the query sequence and tblastn translates all nucleotide sequences in a database, while tblastx translates both the query and the database sequences into amino acid sequences. © 2017 by John Wiley & Sons, Inc.
利用网络BLAST搜索在氨基酸序列中寻找同源物
BLAST，基本本地比对搜索工具，比任何其他生物序列数据库搜索程序使用更频繁。我们将展示如何在Web上运行搜索，并演示如何通过微调特定研究项目的参数来提高性能。我们为解释结果、统计显著性和生物学相关性问题提供指导，并建议进行补充分析。本单元涵盖蛋白质对蛋白质(blastp)搜索和翻译搜索(blastx, tblastn, tfastx)。Blastx从概念上翻译查询序列，tblastn翻译数据库中的所有核苷酸序列，而tblastx将查询序列和数据库序列翻译成氨基酸序列。©2017 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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