[REPRODUCTION OF SCHISTOSOMA MANSONI MOTHER SPOROCYST].

Abstract

The development of generative elements of Schistosoma mansoni mother sporocysts (MS) was examined by histological methods. About 20 large cells, on average, determined as germinal cells (GC) were found in the miracidium. These cells formed a C-shape cellular aggregation (a band) beginning in the caudal part of the larva, and reaching the nerve ganglion in the anterior part. At the level of the 3d tier of epithelial plates of the miracidium, this band shifted to the external body wall, bypassing the zone of excretory channels. Apparently, this shift resulted in the subdivision of a single pool of GC into two structurally associated groups. A group of several undifferentiated cells (UC) was also revealed in the caudal part of the body. After the metamorphosis of the miracidium into sporocysts, GC had increased in size and on the 3d day started to divide, forming first embryos of daughter sporocysts. During the same time, germinal masses were being formed in the subtegumental area of the MS body. Since this time point, proliferation of UC occured only in germinal masses. A part of UC also differentiated there into GC. These cells formed sporocystoid embryos, developing as far as the germinal ball, and then came out into the sporocyst schizocoel (approximately in 10 days p. i.). Thus, in S. mansoni, the formation of generative elements into MS occurs in two stages. Primary GC are formed during the development of the miracidium into the egg, whereas secondary GC develop in germinal masses of the sporocyst.