Arbuscular Mycorrhizal Fungi Increase the Phenolic Compounds Concentration in the Bark of the Stem of Libidibia Ferrea in Field Conditions.

Q3 Immunology and Microbiology
Open Microbiology Journal Pub Date : 2017-10-31 eCollection Date: 2017-01-01 DOI:10.2174/1874285801711010283
Emanuela Lima Dos Santos, Francineyde Alves da Silva, Fábio Sérgio Barbosa da Silva
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引用次数: 20

Abstract

Background: Libidibia ferrea is a species particular to the caatinga presenting medicinal properties for containing bioactive compounds. The use of Arbuscular Mycorrhizal Fungi (AMF) can increase the production of biomolecules in the legume leaves; however, no light has been shed on the role of symbiosis in maximizing metabolites production in the bark of L. ferrea stem.

Objective: The aim was to select AMF that are efficient at increasing the production of phenolic compounds with medicinal properties in the bark of the L. ferrea stem.

Methods: The experiment was designed in randomized blocks with four inoculation treatments (plants pre-inoculated with Claroideoglomus etunicatum, with Gigaspora albida, with Acaulospora longula, and non-inoculated plants - control) with six repetitions. Thirteen months after the transplanting, the plants were pruned and the bark of the stem was collected; subsequently, this plant material was dried in a chamber. After the drying process, fractions of the bark of the stem were macerated in methanol. The extracts were further used for analyses of the biomolecules.

Results: The flavonoids concentration had an increase of, respectively, 236% and 186% in relation to the control for the treatments with A. longula and C. etunicatum; plants inoculated with A. longula had an increase of 47% in total tannins concentration compared with the non-inoculated control - a benefit that the proanthocyanidins did not present.

Conclusion: Applying inoculation with A. longula may be an alternative to increase the production of biomolecules of the secondary metabolism in the bark of the L. ferrea stem in field conditions.

丛枝菌根真菌在田间条件下提高了铁树茎皮中酚类化合物的浓度。
背景:铁利bidibia ferrea是caatinga特有的一种,因其含有生物活性化合物而具有药用价值。利用丛枝菌根真菌(AMF)可以增加豆科植物叶片中生物分子的产量;然而,目前还没有研究表明共生关系在铁属植物茎皮代谢产物最大化中的作用。目的:筛选能有效提高铁氧根茎皮中酚类药用化合物产量的AMF。方法:试验采用随机分组设计,采用4个接种处理(预接种毛囊、粗孢子虫、长孢子虫和未接种对照),每组6次重复。移栽13个月后,修剪植株,收集茎皮;随后,这种植物材料在室内干燥。干燥过程后,茎皮的部分在甲醇中浸泡。提取液进一步用于生物分子分析。结果:与对照相比,龙骨草和弓尾草处理的黄酮类化合物浓度分别提高了236%和186%;与未接种的对照相比,接种了龙葵的植株的总单宁浓度增加了47%,这是原花青素所没有的好处。结论:在田间条件下,接种长曲霉可能是增加铁酸根茎皮次生代谢生物分子产量的一种替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Open Microbiology Journal
Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)
CiteScore
1.80
自引率
0.00%
发文量
24
期刊介绍: The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.
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