Antibiotic Resistance Patterns and Virulence Determinants of Different SCCmec and Pulsotypes of Staphylococcus Aureus Isolated from a Major Hospital in Ilam, Iran.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2017-10-31 eCollection Date: 2017-01-01 DOI:10.2174/1874285801711010211

Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani

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引用次数: 10

Abstract

Aims & objectives: The aim of this study is to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of S. aureus isolated from air, surfaces, staff, and patients in a Public hospital in Ilam.

Methods & materials: A total of 88 of 140 staphylococci identified as S. aureus by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air. The sampling from staff and surfaces was done through using swab and air by standard pump. Antimicrobial susceptibility testing and presence different resistant and virulence determinants was assessed. Isolates were then typed by pulsed-field gel electrophoresis (PFGE) and SCCmec typing methods.

Results: Out of 88isolates, 36 of them (40.9%) were MRSA. Among MRSA isolates, the range of resistance to antibiotic was 0% in vancomycin to 83.3% in gentamycin. The most prevalent resistant genes among gentamicin resistant S. aureus were acc (6')/aph (2")Ia and aph(3")IIIa. The most common erythromycin resistant gene was ermC. Surprisingly, SCCmec types I (30.5%), II (25%)were highly distributed. PFGE analysis showed 33 different pulsotypes.

Conclusion: This study confirms that different isolates of MSSA and MRSA circulate in Ilam which differ in antimicrobial susceptibility, content of resistance, and virulence determinants.

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伊朗伊拉姆一家大医院分离的不同SCCmec和Pulsotypes金黄色葡萄球菌的抗生素耐药模式和毒力决定因素

目的和目的:本研究的目的是评估从伊拉姆一家公立医院的空气、表面、工作人员和患者中分离的不同类型金黄色葡萄球菌的遗传相关性、抗生素耐药性模式和毒力特征。方法与材料:对140株经常规方法和分子方法鉴定为金黄色葡萄球菌的葡萄球菌中的88株进行研究。从表面、工作人员、患者和医院室内空气中获得分离样本。工作人员和表面的采样是通过使用棉签和空气通过标准泵完成的。评估了抗菌素敏感性试验和存在不同的耐药和毒力决定因素。采用脉冲场凝胶电泳(PFGE)和SCCmec分型方法对分离物进行分型。结果:88株分离物中有36株(40.9%)为MRSA。在MRSA分离株中，万古霉素的耐药率为0%，庆大霉素的耐药率为83.3%。耐庆大霉素金黄色葡萄球菌中最常见的耐药基因是acc(6’)/aph(2’)Ia和aph(3’)IIIa。最常见的红霉素耐药基因为ermC。令人惊讶的是，SCCmec I型(30.5%)和II型(25%)分布广泛。PFGE分析显示33种不同的脉冲型。结论:本研究证实了不同分离株的MSSA和MRSA在伊拉姆流行，它们在抗菌药物敏感性、耐药含量和毒力决定因素上存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.