Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells.

Journal of Evidence-Based Complementary & Alternative Medicine Pub Date : 2017-10-01 Epub Date: 2017-07-18 DOI:10.1177/2156587217718751

Helen Mier-Giraldo, Luis Eduardo Díaz-Barrera, Lucy Gabriela Delgado-Murcia, Manuel Fernando Valero-Valdivieso, Gabriela Cáez-Ramírez

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引用次数: 18

Abstract

It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC₅₀) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.

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紫浆果提取物对宫颈癌细胞(HeLa)和成纤维细胞(L929)的细胞毒性和免疫调节活性。

本研究旨在评价熟透青浆果实乙醇和异丙醇粗提物的生物潜力。研究了白细胞介素-6、白细胞介素-8和单核细胞趋化蛋白-1 (MCP-1)表达对人宫颈癌(HeLa)和小鼠成纤维细胞(L929)的细胞毒和免疫调节作用。采用高效液相色谱二极管阵列检测和高效液相色谱紫外/可见光检测对其成分进行鉴定。在两种溶剂中均发现熊果酸和迷迭香酸。而异丙醇提取物中没食子酸、槲皮素和表儿茶素含量较高(P < 0.05)。结果表明，总多酚含量、抗氧化活性和细胞毒活性与溶剂有关。异丙醇提取物对HeLa细胞和L929成纤维细胞的半数最大抑制浓度值(IC50)分别为60.48±3.8 μg/mL和66.62±2.67 μg/mL。提取物降低白细胞介素-6、白细胞介素-8和MCP-1的释放呈剂量依赖性。提取物具有抗癌和免疫调节的潜力，可作为新的补充药物产品的开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Evidence-Based Complementary & Alternative Medicine

自引率

0.00%

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审稿时长

15 weeks

期刊介绍： The Journal of Evidence-Based Integrative Medicine ((JEBIM)), published previously as the Journal of Evidence-Based Complementary and Alternative Medicine (JEBCAM) and also as Complementary Health Practice Review (CHPR). The Journal of Evidence-Based Integrative Medicine (JEBIM) is an interdisciplinary, peer-reviewed, open access, biomedical journal whose aim is to create a global platform for hypothesis-driven and evidence-based research in all fields of integrative medicine. The journal’s objective is to publish papers which impart scientific validity to Integrative Medicine methods that are indispensable and inevitable in today’s world. All papers will be peer reviewed by experts in their respective fields, and papers will be accepted based on their scientific merit. It is the goal of the Journal to help remove the “myth” and provide scientific rationale for the various methodologies and theories of Integrative Medicine. All submissions will be reviewed based on their scientific merit and only papers with sound study design, valid statistical analyses and logical conclusions will be accepted. Topics include, but are not limited to: Traditional Eastern and Western medicine Nutrition therapy and supplementation Massage Therapy Non-traditional treatments Preventative medicine Integrative health and medicine Mindfulness Yoga.