Alexander Z. Harris, Danielle Golder, Ekaterina Likhtik
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引用次数: 11
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Abstract
Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety-related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input-specific and cell-specific contributions to circuit-wide activity. We discuss the planning, execution, and troubleshooting of physiology experiments during anxiety-like behavior. © 2017 by John Wiley & Sons, Inc.
小鼠多位点电生理记录研究焦虑时的跨区域交流
在清醒、自由运动的小鼠中记录神经活动是一种强大而灵活的技术，可用于解剖病理行为背后的神经回路机制。本单元描述了在焦虑相关范例中设计驱动和记录单个神经元和局部场电位的协议。我们还包括整合药理学和光遗传学方法的电路操作方案，当与电生理记录相结合时，证明了输入特异性和细胞特异性对电路全活动的贡献。我们讨论了在类焦虑行为中生理实验的计划、执行和故障排除。©2017 by John Wiley &儿子,Inc。
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