Toxicity of Glandularia selloi (Spreng.) Tronc. leave extract by MTT and neutral red assays: influence of the test medium procedure.

Q3 Environmental Science

Interdisciplinary Toxicology Pub Date : 2016-03-01 Epub Date: 2017-05-17 DOI:10.1515/intox-2016-0004

Luciana Rizzieri Figueiró, Luana Christine Comerlato, Marcia Vignoli Da Silva, José Ângelo Silveira Zuanazzi, Gilsane Lino Von Poser, Ana Luiza Ziulkoski

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引用次数: 7

Abstract

Cytotoxicity assays using cell cultures may be an alternative to assess biological toxicity of plant extracts with potential phytotherapeutic properties. This study compared three methods to prepare culture media for the exposure of Vero cells to plant extracts. Leaves of Glandularia selloi (Spreng.) Tronc. were used to prepare culture medium with aqueous extract, extract in culture medium and methanol extract. Toxicity was assessed using the MTT and neutral red (NR) assays. In general, alterations in the cellular functions were found in all extracts and assays. Cytotoxic effect occurred at lower doses in aqueous extract and the range of effect of the methanol extract was small. The procedure of preparing the test medium has an effect on the outcome of the assay. Cytotoxicity of plant extract can be assessed by MTT and NR assays. Aqueous extract added to the culture medium presented the best profile to assess cytotoxicity.

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麦草的毒性(春季)Tronc。用MTT和中性红测定叶提取物:试验介质程序的影响。

利用细胞培养物进行细胞毒性试验可能是评估具有潜在植物治疗特性的植物提取物生物毒性的一种替代方法。本研究比较了三种制备Vero细胞暴露于植物提取物培养基的方法。草叶(春季)Tronc。分别用水提物、培养基中提物和甲醇提物配制培养基。毒性评估采用MTT和中性红(NR)测定。总的来说，在所有的提取物和分析中都发现了细胞功能的改变。水提物在较低剂量下有细胞毒作用，甲醇提物作用范围较小。制备测试介质的过程对分析的结果有影响。植物提取物的细胞毒性可通过MTT和NR测定来评估。在培养基中加入水提物是评估细胞毒性的最佳方法。

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来源期刊

Interdisciplinary Toxicology Pharmacology, Toxicology and Pharmaceutics-Pharmacology

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18 weeks