The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus

Q2 Medicine

Experimental and Toxicologic Pathology Pub Date : 2017-10-02 DOI:10.1016/j.etp.2017.06.002

Tuğba Kotil , Şengül Şentürk , Türkan Sarioğlu , Leyla Tapul

{"title":"The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus","authors":"Tuğba Kotil , Şengül Şentürk , Türkan Sarioğlu , Leyla Tapul","doi":"10.1016/j.etp.2017.06.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Aims</h3><p>Retinoic acid<span><span> (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and </span>metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.</span></p></div><div><h3>Methods</h3><p>Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40mg/kg/day 13-cis RA for 5<!--> <span><span><span>days by gavage. Uterine tissue sections were treated with </span>BrdU and MMP-2 antibodies, evaluated using </span>light microscopy<span>. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.</span></span></p></div><div><h3>Results</h3><p>MMP-2 immunoreactivity<span><span><span><span> decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the </span>electron microscopy evaluation, the surface epithelial cells of the experimental group showed </span>vacuolization<span>, and an accumulation of lipofuscin<span> bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and </span></span></span>myometrium.</span></p></div><div><h3>Conclusion</h3><p>RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 8","pages":"Pages 643-648"},"PeriodicalIF":0.0000,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.06.002","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Toxicologic Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0940299316302615","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 2

Abstract

Aims

Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.

Methods

Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40 mg/kg/day 13-cis RA for 5 days by gavage. Uterine tissue sections were treated with BrdU and MMP-2 antibodies, evaluated using light microscopy. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.

Results

MMP-2 immunoreactivity decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the electron microscopy evaluation, the surface epithelial cells of the experimental group showed vacuolization, and an accumulation of lipofuscin bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and myometrium.

Conclusion

RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.

查看原文本刊更多论文

维甲酸对大鼠子宫mmp-2生成、增殖及超微结构形态的影响

目的维甲酸(RA)对于保证许多组织的连续性和形态具有至关重要的作用。基质金属蛋白酶(MMPs)在肿瘤的增殖、形成和转移中具有重要作用。本研究探讨了RA对大鼠子宫细胞MMP-2生成的影响。方法将24只成年Spraque Dawley大鼠分为两组，实验组给予40 mg/kg/d 13-cis RA灌胃5 d;子宫组织切片用BrdU和MMP-2抗体处理，光镜下评估。2.5%戊二醛固定组织，透射电镜观察。结果实验组间质细胞smmp -2免疫反应性较对照组降低，腺上皮未见MMP-2染色。与对照组相比，ra处理组细胞的BrDU标记显著降低。电镜观察，实验组表面上皮细胞呈空泡化，腺体上皮内可见脂褐素小体堆积。涉及自噬空泡的细胞在整个子宫层中含有过量的脂质颗粒，特别是在子宫内膜和子宫肌层的边界。结论ra能抑制MMP-2的表达，对细胞增殖和细胞形态有负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Experimental and Toxicologic Pathology 医学-病理学

CiteScore

2.08

自引率

0.00%

发文量

审稿时长

5.3 weeks

期刊介绍： Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.