Experimental Evaluation of Hiṅgvādi Ghṛta in Behavioral Despair Using Animal Models.

Poonam Ashish Gupte, Jayshree Dawane, Asmita Ashish Wele
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引用次数: 3

Abstract

Context: Depression, a sustained mood disorder caused by selective diminution of specialized cells in brain is increasing at an alarming rate. It will be the second largest morbid illness by next decade and is the leading cause of suicidal deaths. The available antidepressant medications benefit only a third of its recipients and have many side effects. Hence, it is imperative to search in Ayurveda for leads.

Aim: To evaluate Anti- depressant activity of Hiṅgvādi Ghṛta in vivo .

Settings and design: Comparative preclinical study.

Materials and methods: Hiṅgvādi Ghṛta (HG) was prepared using standard operating procedure, physicochemically analyzed and assessed. Tail Suspension Test (TST) model with Swiss albino mice and Forced Swim Test (FST) model with Wistar albino rats were used to assess anti-depressant activity. Imipramine hydrochloride in dose of 15 mg/kg for TST and 10 mg/kg for FST, was the standard drug and Ghee as vehicle control in dose of 0.1g/20g for TST and 0.72g/200g for FST orally. Hiṅgvādi Ghṛta in doses of 0.05g/20g (x/2), 0.1g/20g (x) and 0.2 g/20g (2x) for TST and 0.36g/200g (x/2), 0.72g/200g (x) and 1.44g/200g (2x) for FST was administered to 3 test groups for 21 days orally except Plain control group which received only distilled water. Duration of immobility in seconds for TST and number of rotations for FST were noted for assessment.

Statistical analysis used: One way ANOVA followed by Dunnets test and Paired t test.

Results: HG was significantly effective at dose of 0.1gm/20gm for TST (P = 0.0037; P < 0.01) and 0.72g/200g for FST (P = 0.0055, P < 0.01) comparable to Imipramine hydrochloride.

Conclusions: HG displayed potent anti depressant activity comparable to standard drug Imipramine Hydrochloride.

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使用动物模型对Hiṅgvādi Ghṛta在行为绝望中的实验评价。
背景:抑郁症,一种由大脑特定细胞选择性减少引起的持续情绪障碍,正在以惊人的速度增加。到下一个十年,它将成为第二大病态疾病,也是自杀死亡的主要原因。现有的抗抑郁药物只能使三分之一的患者受益,而且有许多副作用。因此,在阿育吠陀中寻找线索是必要的。目的:评价Hiṅgvādi Ghṛta的体内抗抑郁活性。设置与设计:临床前比较研究。材料和方法:Hiṅgvādi Ghṛta (HG)采用标准操作程序制备,理化分析和评价。采用瑞士白化小鼠尾部悬吊试验(TST)模型和Wistar白化大鼠强迫游泳试验(FST)模型评估抗抑郁活性。以盐酸丙咪嗪为标准药,TST剂量为15 mg/kg, FST剂量为10 mg/kg, Ghee为对照药,TST剂量为0.1g/20g, FST剂量为0.72g/200g。除普通对照组只给予蒸馏水外,其余3个实验组分别以TST 0.05g/20g (x/2)、0.1g/20g (x)、0.2 g/20g (2x)的Hiṅgvādi Ghṛta剂量和FST 0.36g/200g (x/2)、0.72g/200g (x)、1.44g/200g (2x)的Hiṅgvādi Ghṛta剂量口服21 d。TST的静止时间(以秒为单位)和FST的旋转次数被记录下来以供评估。统计分析采用单因素方差分析,随后采用Dunnets检验和配对t检验。结果:HG在0.1gm/20gm剂量下对TST有显著疗效(P = 0.0037;FST为0.72g/200g (P = 0.0055, P < 0.01),与盐酸丙咪嗪相当。结论:HG具有与标准药物盐酸丙咪嗪相当的抗抑郁活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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