Chemical genomic analysis of GPR35 signaling.

IF 1.4
Heidi Haibei Hu, Huayun Deng, Shizhang Ling, Haiyan Sun, Terry Kenakin, Xinmiao Liang, Ye Fang
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引用次数: 6

Abstract

GPR35, a family A orphan G protein-coupled receptor, has been implicated in inflammatory, neurological, and cardiovascular diseases. However, not much is known about the signaling and functions of GPR35. We performed a label-free kinome short hairpin RNA screen and identified a putative signaling network of GPR35 in HT-29 cells, some of which was validated using gene expression, biochemical and cellular assays. The results showed that GPR35 induced hypoxia-inducible factor 1α, and was involved in synaptic transmission, sensory perception, the immune system, and morphogenetic processes. Collectively, our data suggest that GPR35 may play an important role in response to hypoxic stress and be a potential target for the treatment of inflammatory, cardiovascular, and neurological disorders.

GPR35信号传导的化学基因组分析。
GPR35是一种a家族孤儿G蛋白偶联受体,与炎症、神经系统和心血管疾病有关。然而,对GPR35的信号传导和功能知之甚少。我们进行了无标记的kinome短发夹RNA筛选,并在HT-29细胞中发现了一个假设的GPR35信号网络,其中一些通过基因表达、生化和细胞分析得到了验证。结果表明,GPR35诱导缺氧诱导因子1α,参与突触传递、感觉知觉、免疫系统和形态发生过程。总的来说,我们的数据表明GPR35可能在应对缺氧应激中发挥重要作用,并可能成为治疗炎症、心血管和神经系统疾病的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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