The Ginkgo biloba extract (GbE) protects the kidney from damage produced by a single and low dose of carbon tetrachloride in adult male rats

Q2 Medicine

Experimental and Toxicologic Pathology Pub Date : 2017-09-05 DOI:10.1016/j.etp.2017.04.003

R.M. Chávez-Morales , F. Jaramillo-Juárez , M.L. Rodríguez-Vázquez , M.C. Martínez-Saldaña , F.A. Posadas del Río , J.A. Garfias-López

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引用次数: 17

Abstract

Gingko biloba leaves have been used as herbal medicine in China for 5000 years, and the standardized leaf extract (GB-STE) has some beneficial effects in the treatment of age-related, cardiovascular, and neurological diseases. The aim of this study was to investigate the renoprotective effects of the Gingko biloba extract (GbE) against the toxicity of a single and relatively low dose of carbon tetrachloride (CCl₄). In male adult Wistar rats, we determined the urine flux, the concentration of total proteins in urine, the concentration of glucose in urine, and the concentration of malondialdehyde (MDA) in renal cortex as well as two markers of renal function (clearance of inulin and p-aminohippurate); we also compared the histological lesions caused by CCl₄. Carbon tetrachloride increased the urinary concentration of total proteins, and the renal concentration of MDA; however, it did not modify the urine flux, urinary concentration of glucose, nor the inuline or the p-aminohipurate clearances. Morphologically, CCl₄ generated some tubular damage that was more intense in the inner cortex of kidneys. The GbE extract counteracted the effects of CCl₄ on the concentration of total proteins in urine, the concentration of renal MDA, and the renal histological changes. In conclusion the main toxic effects produced by CCl₄ were prevented by the GbE, probably due to their antioxidant properties and the inhibition of the main P450 isoenzyme (CYP2E1) that metabolize CCl₄.

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银杏叶提取物(GbE)可以保护成年雄性大鼠的肾脏免受单次和低剂量四氯化碳造成的损害

银杏叶在中国作为草药已有5000年的历史，其标准化叶提取物(GB-STE)在治疗年龄相关疾病、心血管疾病和神经系统疾病方面具有一定的有益作用。本研究的目的是研究银杏叶提取物(GbE)对单一和相对低剂量四氯化碳(CCl4)毒性的肾保护作用。测定雄性成年Wistar大鼠的尿通量、尿中总蛋白浓度、尿中葡萄糖浓度、肾皮质丙二醛(MDA)浓度以及两种肾功能指标(菊粉清除率和对氨基马粪酸清除率);我们还比较了CCl4引起的组织学病变。四氯化碳使尿总蛋白浓度和肾MDA浓度升高;然而，它并没有改变尿通量、尿葡萄糖浓度，也没有改变菊胺或对氨基hipurate的清除率。形态学上，CCl4对肾内皮质造成一定程度的肾小管损伤。GbE提取物可以抵消CCl4对大鼠尿液总蛋白浓度、肾脏MDA浓度和肾脏组织学改变的影响。综上所述，GbE可以抑制CCl4产生的主要毒性作用，这可能是由于GbE的抗氧化特性和对代谢CCl4的主要P450同工酶(CYP2E1)的抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental and Toxicologic Pathology 医学-病理学

CiteScore

2.08

自引率

0.00%

发文量

审稿时长

5.3 weeks

期刊介绍： Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.