Methods for Quantitative Creatinine Determination

Current Protocols in Human Genetics Pub Date : 2017-04-06 DOI:10.1002/cphg.38

John F. Moore, J. Daniel Sharer

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引用次数: 35

Abstract

Reliable measurement of creatinine is necessary to assess kidney function, and also to quantitate drug levels and diagnostic compounds in urine samples. The most commonly used methods are based on the Jaffe principal of alkaline creatinine-picric acid complex color formation. However, other compounds commonly found in serum and urine may interfere with Jaffe creatinine measurements. Therefore, many laboratories have made modifications to the basic method to remove or account for these interfering substances. This appendix will summarize the basic Jaffe method, as well as a modified, automated version. Also described is a high performance liquid chromatography (HPLC) method that separates creatinine from contaminants prior to direct quantification by UV absorption. Lastly, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described that uses stable isotope dilution to reliably quantify creatinine in any sample. This last approach has been recommended by experts in the field as a means to standardize all quantitative creatinine methods against an accepted reference. © 2017 by John Wiley & Sons, Inc.

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定量测定肌酐的方法

可靠的肌酐测量对于评估肾功能、定量药物水平和尿液样本中的诊断性化合物是必要的。最常用的方法是基于贾菲原理的碱性肌酐-苦味酸络合物显色法。然而，血清和尿液中常见的其他化合物可能会干扰Jaffe肌酐测量。因此，许多实验室对基本方法进行了修改，以去除或解释这些干扰物质。本附录将总结基本的Jaffe方法，以及修改后的自动化版本。还描述了一种高效液相色谱(HPLC)方法，该方法在通过紫外吸收直接定量之前将肌酐从污染物中分离出来。最后，描述了一种液相色谱-串联质谱(LC-MS/MS)方法，该方法使用稳定同位素稀释来可靠地定量任何样品中的肌酐。最后一种方法已被该领域的专家推荐为标准化所有定量肌酸酐方法的一种手段。©2017 by John Wiley &儿子,Inc。

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Current Protocols in Human Genetics Biochemistry, Genetics and Molecular Biology-Genetics

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期刊介绍： Current Protocols in Human Genetics is the resource for designing and running successful research projects in all branches of human genetics.