Amrendra Kumar, Jelena S. Bezbradica, Aleksandar K. Stanic, Sebastian Joyce
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引用次数: 9
Abstract
Semi-invariant natural killer T (iNKT) cells are CD1d-restricted innate-like lymphocytes that recognize lipid agonists. Activated iNKT cells have immunoregulatory properties. Human and mouse iNKT cell functions elicited by different glycolipid agonists are highly conserved, making the mouse an excellent animal model for understanding iNKT cell biology in vivo. This unit describes basic methods for the characterization and quantification (see Basic Protocol 1) and functional analysis of mouse iNKT cells in vivo or in vitro. This unit also contains protocols that describe enrichment and purification of iNKT cells, generation of CD1d tetramer, and lipid antigen loading onto cell-bound and soluble CD1d for activation of NKT cell hybridomas. © 2017 by John Wiley & Sons, Inc.
小鼠半不变天然T细胞的特性及功能分析
半不变自然杀伤T (iNKT)细胞是cd1限制性先天样淋巴细胞，可识别脂质激动剂。活化的iNKT细胞具有免疫调节特性。不同糖脂激动剂诱导的人和小鼠iNKT细胞功能高度保守，使小鼠成为了解iNKT细胞生物学的良好动物模型。本单元描述了小鼠iNKT细胞在体内和体外的表征和定量的基本方法(见基本方案1)和功能分析。该单元还包含描述iNKT细胞的富集和纯化，CD1d四聚体的产生，以及脂质抗原加载到细胞结合和可溶性CD1d上以激活NKT细胞杂交瘤的方案。©2017 by John Wiley &儿子,Inc。
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