[EXPRESSION OF PFKFB, HK2, NAMPT, TSPAN13 AND HSPB8 GENES IN PEDIATRIC GLIOMA].

Likars'ka sprava Pub Date : 2015-10-01

D O Minchenko, Y E Novik, H S Maslak, O V Tiazhka, O H Minchenko

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Abstract

We studied the peculiarity of the expression of several key genes related to dysregulation of cell proliferation and surviving processes in pediatric glioma (glioblastoma multiforme) tissue from five children with age from 5 to 8 years as well a sin corresponding nonmalignant tissue counterparts as control from the same patients. RNA was isolated from glioma tissue and corresponding non-malignant tissue counterparts and PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 gene expressions were studied by quantitative polymerase chain reaction. It was shown that the expression level of genes PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 is increased in pediatric gliomas as compared to corresponding non-malignant tissue counterparts, but in different grade. More significant changes were demonstrated for PFKFB3, PFKFB4 HK2, NAMPT, TSPAN13, and HSPB8 genes. Thus, the changes in pediatric glioma tissues of the expression of PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 genes, which control cell proliferation and apoptosis, possibly contribute to enhance the tumor growth, because these genes control cell proliferation and surviving.

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[pfkfb、hk2、nampt、tspan13和hspb8基因在小儿胶质瘤中的表达]。

我们研究了来自5名5 - 8岁儿童的小儿胶质瘤(多形性胶质母细胞瘤)组织中与细胞增殖和存活过程失调相关的几个关键基因的表达特性，以及来自同一患者的相应非恶性组织对照。从胶质瘤组织和相应的非恶性组织中分离RNA，通过定量聚合酶链反应研究PFKFB1、PFKFB2、PFKFB3、PFKFB4、HK2、NAMPT、TSPAN13和HSPB8基因的表达。结果表明，在小儿胶质瘤中，基因PFKFB1、PFKFB2、PFKFB3、PFKFB4、HK2、NAMPT、TSPAN13和HSPB8的表达水平比相应的非恶性组织中相应基因的表达水平升高，但表达程度不同。PFKFB3、PFKFB4、HK2、NAMPT、TSPAN13和HSPB8基因的变化更为显著。因此，在小儿胶质瘤组织中，控制细胞增殖和凋亡的基因PFKFB1、PFKFB2、PFKFB3、PFKFB4、HK2、NAMPT、TSPAN13和HSPB8的表达变化可能有助于促进肿瘤的生长，因为这些基因控制细胞的增殖和存活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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