Intestinal Preparation Techniques for Histological Analysis in the Mouse

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2016-06-01 DOI:10.1002/cpmo.2

Jonathan M. Williams, Carrie A. Duckworth, Kate Vowell, Michael D. Burkitt, D. Mark Pritchard

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引用次数: 47

Abstract

The murine intestinal tract represents a difficult organ system to study due to its long convoluted tubular structure, narrow diameter, and delicate mucosa which undergoes rapid changes after sampling prior to fixation. These features do not make for easy histological analysis as rapid fixation in situ, or after simple removal without careful dissection, results in poor postfixation tissue handling and limited options for high quality histological sections. Collecting meaningful quantitative data by analysis of this tissue is further complicated by the anatomical changes in structure along its length. This article describes two methods of intestinal sampling at necropsy that allow systematic histological analysis of the entire intestinal tract, either through examination of cross sections (circumferences) by the gut bundling technique or longitudinal sections by the adapted Swiss roll technique, together with basic methods for data collection. © 2016 by John Wiley & Sons, Inc.

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小鼠肠道制备技术的组织学分析

小鼠肠道是一个难以研究的器官系统，由于其长而卷曲的管状结构，狭窄的直径和脆弱的粘膜在固定前取样后发生快速变化。这些特征使得原位快速固定或未经仔细剥离的简单切除后不易进行组织学分析，导致固定后组织处理不良，高质量组织学切片的选择有限。通过分析该组织收集有意义的定量数据进一步复杂化了沿其长度结构的解剖变化。这篇文章描述了两种尸检时的肠道取样方法，可以对整个肠道进行系统的组织学分析，要么通过肠束技术检查横切面(周长)，要么通过采用瑞士卷技术检查纵切面，以及数据收集的基本方法。©2016 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.