An Overview of Mouse Models of Nonalcoholic Steatohepatitis: From Past to Present

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2016-06-01 DOI:10.1002/cpmo.3

Ans Jacobs, Anne-Sophie Warda, Jef Verbeek, David Cassiman, Pieter Spincemaille

引用次数: 34

Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the Western world. It is associated with obesity and type 2 diabetes and represents a spectrum of histological abnormalities ranging from simple steatosis to non-alcoholic steatohepatitis (NASH), which can further progress to fibrosis, cirrhosis, hepatocellular carcinoma (HCC), and liver failure. To gain insight into the pathogenesis and evaluate treatment options, mouse models of NAFLD/NASH are of utmost importance. There is a high phenotypical variety in the available mouse models, however, models that truly display the full spectrum of histopathological and metabolic features associated with human NASH are rare. In this review, we summarize the most important NAFLD/NASH mouse models that have been developed over the years and briefly highlight the pros and cons. Also, we illustrate the preclinical research in which these models have been used. © 2016 by John Wiley & Sons, Inc.

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非酒精性脂肪性肝炎小鼠模型综述:从过去到现在

非酒精性脂肪性肝病(NAFLD)是西方世界最常见的慢性肝病。它与肥胖和2型糖尿病有关，并表现为一系列组织学异常，从单纯脂肪变性到非酒精性脂肪性肝炎(NASH)，后者可进一步发展为纤维化、肝硬化、肝细胞癌(HCC)和肝功能衰竭。为了深入了解发病机制和评估治疗方案，NAFLD/NASH的小鼠模型至关重要。在现有的小鼠模型中有很高的表型多样性，然而，真正显示与人类NASH相关的全谱组织病理学和代谢特征的模型是罕见的。在这篇综述中，我们总结了近年来开发的最重要的NAFLD/NASH小鼠模型，并简要强调了其优缺点。此外，我们还说明了这些模型已被使用的临床前研究。©2016 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.