Effect of methyl-beta-cyclodextrin on the viability and acrosome damage of sex-sorted sperm in frozen-thawed bovine semen.

IF 1.9 3区生物学 Q2 BIOLOGY

Journal of Biological Research-Thessaloniki Pub Date : 2016-04-12 eCollection Date: 2016-12-01 DOI:10.1186/s40709-016-0043-x

Seunghyung Lee, Yong-Seung Lee, Sang-Hee Lee, Boo-Keun Yang, Choon-Keun Park

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引用次数: 3

Abstract

Background: The regulation of methyl-beta-cyclodextrin (MBCD) on cryodamage on X- and Y-sperm during cryopreservation of semen was investigated. The semen was collected from ten healthy bulls of proven fertility by an artificial vagina. The bovine sperm treated with MBCD fresh solution (0, 1, 5, 10, and 20 mM). The sperms were evaluated for viability and acrosome damage using flow cytometry. Moreover, X- and Y-sperm in frozen-thawed bovine semen were sorted by flow cytometry after Hoechst 33342-dyed, and the viability and acrosome damage of sperms were analyzed.

Results: Sperm viability in frozen-thawed semen was decreased by MBCD (p < 0.05), also the acrosome damage of sperm was significantly increased (p < 0.05). Moreover, we sorted X- and Y-sperm from frozen-thawed bovine semen for observing the viability and acrosome damage on the separated X- and Y-sperm after MBCD treatment. Viability of X-sperm was significantly lower than that of Y-sperm (p < 0.05). Also, acrosome damage of X-sperm was significantly higher than Y-sperm (p < 0.05).

Conclusions: Methyl-beta-cyclodextrin enhances damage of sperm in frozen-thawed bovine semen, and X-sperm is more sensitive than Y-sperm in cell damage. These results demonstrate that MBCD can inhibit viability of spermatozoa in frozen-thawed bovine semen (for X-sperm, especially).

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甲基- β -环糊精对冻融牛精液中性别分选精子活力和顶体损伤的影响。

背景:研究了甲基- β -环糊精(MBCD)在精液冷冻保存过程中对X-和y -精子冷冻损伤的调节作用。精液是通过人工阴道从10头经证实有生育能力的健康公牛身上采集的。牛精子用MBCD新鲜溶液(0、1、5、10和20 mM)处理。用流式细胞术评估精子活力和顶体损伤。用Hoechst 33342染色后的牛冻融精液进行X-和y -精子的流式细胞术分选，分析精子活力和顶体损伤情况。结论:甲基- β -环糊精能增强牛冻融精液中精子的损伤作用，且x精子比y精子对细胞损伤更敏感。这些结果表明，MBCD可以抑制冻融牛精液(尤其是x精子)中精子的活力。

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来源期刊

Journal of Biological Research-Thessaloniki 生物-生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.