Teratogenic Effect of Crude Ethanolic Root Bark and Leaf Extracts of Rauwolfia vomitoria (Apocynaceae) on Nissl Substances of Albino Wistar Rat Fetuses.

Neuroscience journal Pub Date : 2013-01-01 Epub Date: 2013-03-24 DOI:10.1155/2013/906731
Mokutima A Eluwa, Theresa B Ekanem, Paul B Udoh, Moses B Ekong, Olaitan R Asuquo, Amabe O Akpantah, Agnes O Nwakanma
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引用次数: 6

Abstract

Rauwolfia vomitoria is a plant used for the treatment of insanity. The possible adverse effects of crude ethanolic root bark and leaf extract of the plant on Nissl substances of albino Wistar rat fetuses were studied using 25 mature female Wistar rats. The animals were divided equally into 5 groups, labeled A, B, C, D, and E. Group A was the control, while groups B, C, D, and E were the experimental. The female rats were mated with the males overnight, and the sperm positive day was designated as day zero of pregnancy. Oral doses of 150 mg/kg and 250 mg/kg body weight of the root bark extract were administered to groups B and C animals, respectively, while groups D and E animals received 150 mg/kg and 250 mg/kg body weight of the leaf extract, respectively, from day 7 to 11 of gestation. On day 20 of gestation, the rats were sacrificed, the fetuses brains extracted, and the cerebral cortices excised and routinely processed for Nissl substances using Cresyl fast violet staining method. Results showed reduced staining intensity of Nissl substances in the treated groups, especially those that received the root extract. Thus, the herbs may have adverse effects on protein synthesis within the cerebral cortex.

Abstract Image

Abstract Image

罗布麻粗乙醇根皮及叶提取物对白化Wistar大鼠胎尼氏物质的致畸作用。
牛油果是一种用来治疗精神错乱的植物。以25只成熟雌性Wistar大鼠为实验对象,研究了该植物粗乙醇根皮和叶提取物对白化Wistar大鼠胎儿尼氏物质可能产生的不良影响。将动物平均分为A、B、C、D、E 5组,A组为对照组,B、C、D、E组为实验组。雌性大鼠与雄性大鼠在夜间交配,精子阳性的一天被指定为怀孕的第0天。B组和C组分别口服150 mg/kg和250 mg/kg体重的根皮提取物,D组和E组分别口服150 mg/kg和250 mg/kg体重的根皮提取物,试验时间为妊娠第7 ~ 11天。妊娠第20天处死大鼠,提取胎脑,切除大脑皮层,用甲酚快紫染色法常规处理尼氏物质。结果显示,各处理组尼氏物质染色强度降低,尤以根提取物组为明显。因此,这些草药可能对大脑皮层内的蛋白质合成有不利影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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