[The Stimulating Effect of Exometabolites of the Marine Microalgae Phaeodactylum tricornutum Bohlin on Reproduction of Listeria monocytogenes].

Abstract

The biological activity of ethyl acetate extract of exometabolites from the marine microalgae Phaeodactylum tricornutum against a test culture of Listeria monocytogenes 4b has been investigated. It was shown that the stimulating effect of algal exometabolites on the growth of pathogenic bacteria increased maximally by 98.3% on day 6 of the cultivation, if the active substances comprising the culture liquid of P. tricornutum were separated by successive extractions with solvents in order of increasing polarity (hexane, benzene, ethyl acetate), and by 150% on day 3, if the substances from the ethyl acetate extract of exometabolites were separated by column chromatography. It is noted that the fraction of biologically active substances maximally stimulating the growth of L. monocytogenes can be used to prepare an accumulation medium for detecting these patho- genic bacteria in marine environments and hydrobionts.