Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches

Pathologie-biologie Pub Date : 2015-09-01 DOI:10.1016/j.patbio.2015.07.007

S. Alibi , A. Ferjani , O. Gaillot , M. Marzouk , R. Courcol , J. Boukadida

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引用次数: 38

Abstract

We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) for the identification of 97 Corynebacterium clinical in comparison to identification strains by Api Coryne and MALDI-TOF-MS using 16S rRNA gene and hypervariable region of rpoB genes sequencing as a reference method. C. striatum was the predominant species isolated followed by C. amycolatum. There was an agreement between Api Coryne strips and MALDI-TOF-MS identification in 88.65% of cases. MALDI-TOF-MS was unable to differentiate C. aurimucosum from C. minutissimum and C. minutissimum from C. singulare but reliably identify 92 of 97 (94.84%) strains. Two strains remained incompletely identified to the species level by MALDI-TOF-MS and molecular approaches. They belonged to Cellulomonas and Pseudoclavibacter genus. In conclusion, MALDI-TOF-MS is a rapid and reliable method for the identification of Corynebacterium species. However, some limits have been noted and have to be resolved by the application of molecular methods.

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应用Api Coryne、maldi - tof质谱和分子方法鉴定临床相关棒状杆菌菌株

以16S rRNA基因和rpoB基因高变区测序为参比方法，将Bruker Biotyper基质辅助激光解吸电离飞行时间(MALDI-TOF)质谱(MS)技术用于97株棒状杆菌临床鉴定，并与Api Coryne和MALDI-TOF-MS鉴定菌株进行比较。纹状体为优势种，其次为扁桃。88.65%的病例Api Coryne试纸与MALDI-TOF-MS鉴定吻合。MALDI-TOF-MS不能区分金黄色葡萄球菌和微小葡萄球菌，微小葡萄球菌和奇异葡萄球菌，但能可靠鉴别97株中的92株(94.84%)。MALDI-TOF-MS和分子鉴定方法仍未完全鉴定出两株菌株。它们属于纤维素单胞菌属和假锁菌属。综上所述，MALDI-TOF-MS是一种快速、可靠的棒状杆菌种类鉴定方法。然而，已经注意到一些限制，必须通过应用分子方法来解决。

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来源期刊

Pathologie-biologie 医学-病理学

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0.00%

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审稿时长

6-12 weeks