Diagnostic utility of immunohistochemical markers alpha methyl acyl coA racemase (AMACR) and Ets related gene (ERG) in prostate cancer.

IF 1.1 Q4 ONCOLOGY

International journal of clinical and experimental pathology Pub Date : 2022-09-15 eCollection Date: 2022-01-01

Norton Stephen, Bhawana A Badhe

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Abstract

Objectives: To study the sensitivity and specificity of IHC markers AMACR and ERG in prostatic adenocarcinoma.

Methods: The study was a prospective one and samples were collected from August 2014 to June 2016. A total of 186 samples were obtained from the Department of Urology, in which 112 of these were benign prostatic hyperplasia (BPH), and 71 were prostatic adenocarcinoma. The adenocarcinoma cases were evaluated by two histopathologists, and appropriate Gleason score was given according to the modified ISUP Gleason grading system (2016). IHC markers AMACR & ERG were performed on the adenocarcinoma cases and their sensitivity and specificity were calculated.

Results: AMACR was a highly sensitive and specific marker for detecting prostatic carcinoma with a sensitivity and specificity of 95.8% and 96.5% respectively. ERG was a very specific marker with poor sensitivity in detecting prostate cancer. The sensitivity and specificity of ERG were 35.2% and 100% respectively. ERG expression decreased with increasing Gleason grade, PSA level, and tumour volume, which was statistically significant while the association of AMACR with Gleason grade or with tumor volume was not significant.

Conclusion: ERG is a marker of early prostatic carcinogenesis and tumors may be positive or negative subtypes. Special histomorphologic features like perineural invasion, glomerulations, and intraluminal blue mucin were also studied. AMACR was a highly sensitive marker for detecting prostatic adenocarcinoma, while ERG was highly specific.

本刊更多论文

免疫组织化学标记α甲基酰基辅酶a消旋酶(AMACR)和Ets相关基因(ERG)在前列腺癌中的诊断价值

目的:探讨免疫组化标志物AMACR和ERG在前列腺腺癌中的敏感性和特异性。方法:本研究为前瞻性研究，于2014年8月至2016年6月采集样本。泌尿科共收集186例标本，其中良性前列腺增生112例，前列腺腺癌71例。腺癌病例由两名组织病理学家评估，并根据改进的ISUP Gleason评分系统(2016)给出适当的Gleason评分。对腺癌患者进行免疫组化标记物AMACR和ERG检测，计算其敏感性和特异性。结果:AMACR检测前列腺癌具有较高的敏感性和特异性，敏感性和特异性分别为95.8%和96.5%。ERG是一种特异性很强的标志物，但检测前列腺癌的敏感性较差。ERG的敏感性为35.2%，特异性为100%。ERG表达随Gleason分级、PSA水平和肿瘤体积的增加而降低，差异有统计学意义，而AMACR与Gleason分级和肿瘤体积的相关性无统计学意义。结论:ERG是前列腺早期癌变的标志物，肿瘤可分为阳性亚型和阴性亚型。特殊的组织形态学特征，如神经周围浸润，肾小球和腔内蓝色粘蛋白也进行了研究。AMACR是检测前列腺腺癌的高度敏感的标志物，而ERG是高度特异性的标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International journal of clinical and experimental pathology ONCOLOGY-PATHOLOGY

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0.00%

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审稿时长

1 months

期刊介绍： The International Journal of Clinical and Experimental Pathology (IJCEP, ISSN 1936-2625) is a peer reviewed, open access online journal. It was founded in 2008 by an international group of academic pathologists and scientists who are devoted to the scientific exploration of human disease and the rapid dissemination of original data. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal.