Prognostic value of Talin-1 in renal cell carcinoma and its association with B7-H3.

IF 1.9
Leili Saeednejad Zanjani, Somayeh Vafaei, Maryam Abolhasani, Fahimeh Fattahi, Zahra Madjd
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引用次数: 4

Abstract

Methods: Talin-1 protein was demonstrated as a potential prognostic marker in renal cell carcinoma (RCC) using bioinformatics analysis. We, therefore, examined the protein expression levels and prognostic significance of Talin-1 with a clinical follow-up in a total of 269 tissue specimens from three important subtypes of RCC and 30 adjacent normal samples using immunohistochemistry. Then, we used combined analysis with B7-H3 to investigate higher prognostic values.

Results: The results showed that high membranous and cytoplasmic expression of Talin-1 was significantly associated with advanced nucleolar grade, microvascular invasion, histological tumor necrosis, and invasion to Gerota's fascia in clear cell RCC (ccRCC). In addition, high membranous and cytoplasmic expression of Talin-1 was found to be associated with significantly poorer disease-specific survival (DSS) and progression-free survival (PFS). Moreover, increased cytoplasmic expression of Talin-1High/B7-H3High compared to the other phenotypes was associated with tumor aggressiveness and progression of the disease, and predicted a worse clinical outcome, which may be an effective biomarker to identify ccRCC patients at high risk of recurrence and metastasis.

Conclusions: Collectively, these observations indicate that Talin-1 is an important molecule involved in the spread and progression of ccRCC when expressed particularly in the cytoplasm and may serve as a novel prognostic biomarker in this subtype. Furthermore, a combined analysis of Talin-1/B7-H3 indicated an effective biomarker to predict the progression of disease and prognosis in ccRCC.

Talin-1在肾细胞癌中的预后价值及其与B7-H3的关系。
方法:应用生物信息学分析证实Talin-1蛋白是肾癌(RCC)的潜在预后标志物。因此,我们利用免疫组织化学技术,对来自三种重要亚型RCC的269个组织标本和30个相邻正常样本进行了临床随访,检测了Talin-1的蛋白表达水平和预后意义。然后,我们使用B7-H3联合分析来研究更高的预后价值。结果:在透明细胞RCC (ccRCC)中,Talin-1在膜质和细胞质中的高表达与晚期核仁分级、微血管侵袭、组织学肿瘤坏死和侵犯Gerota’s筋膜显著相关。此外,发现Talin-1的高膜质和细胞质表达与较差的疾病特异性生存(DSS)和无进展生存(PFS)显著相关。此外,与其他表型相比,细胞质中Talin-1High/B7-H3High的表达增加与肿瘤的侵袭性和疾病的进展相关,并预示着更差的临床结果,这可能是识别复发和转移高风险的ccRCC患者的有效生物标志物。结论:总的来说,这些观察结果表明Talin-1是参与ccRCC扩散和进展的重要分子,特别是在细胞质中表达,并且可能作为该亚型的新型预后生物标志物。此外,Talin-1/B7-H3的联合分析表明,Talin-1/B7-H3是预测ccRCC疾病进展和预后的有效生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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