Observations on chromosome-specific sequencing for the construction of cross-species chromosome homology maps and its resolution of human:alpaca homology.

IF 1.3 4区生物学 Q4 GENETICS & HEREDITY

Molecular Cytogenetics Pub Date : 2022-10-07 DOI:10.1186/s13039-022-00622-0

Malcolm A Ferguson-Smith, Jorge C Pereira, Ana Borges, Fumio Kasai

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引用次数: 0

Abstract

Background: The history of comparative chromosome mapping is briefly reviewed, with discussion about the problem that occurs in chromosome painting when size heteromorphisms between homologues cause contamination in chromosomes sorted by flow cytometry that are used in the preparation of chromosome-specific DNA probes.

Main body: As an example, we show in the alpaca (Vicagna pacos) that sequencing of contaminated chromosome sorts can reveal chromosome homologies from alignment with human and mouse genome reference sequences. The procedure identifies syntenic blocks of DNA separated in the human karyotype that are associated in the closely related alpaca and dromedary (Camelus dromedarius) karyotypes. This example provides proof of principal for the validity of the method for comparative chromosome mapping.

Conclusion: It is suggested that the approach presented here may have application in the construction of comparative chromosome maps between distantly related taxa, such as monotremes and mammals.

Abstract Image

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跨物种染色体同源图谱构建及人羊驼同源性的染色体特异性测序观察。

背景:简要回顾了比较染色体作图的历史，并讨论了在制备染色体特异性DNA探针时，同源物之间的大小异型导致流式细胞术分类的染色体受到污染时在染色体绘制中出现的问题。正文:作为一个例子，我们在羊驼(Vicagna pacos)中展示了受污染的染色体排序可以通过与人类和小鼠基因组参考序列比对来揭示染色体同源性。该程序鉴定了在人类核型中分离的DNA的合成块，这些DNA与密切相关的羊驼和单峰骆驼核型有关。这个例子为比较染色体作图方法的有效性提供了原理证明。结论:该方法可应用于单孔目动物和哺乳动物等远亲类群的比较染色体图谱的构建。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Cytogenetics GENETICS & HEREDITY-

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Cytogenetics encompasses all aspects of chromosome biology and the application of molecular cytogenetic techniques in all areas of biology and medicine, including structural and functional organization of the chromosome and nucleus, genome variation, expression and evolution, chromosome abnormalities and genomic variations in medical genetics and tumor genetics. Molecular Cytogenetics primarily defines a large set of the techniques that operate either with the entire genome or with specific targeted DNA sequences. Topical areas include, but are not limited to: -Structural and functional organization of chromosome and nucleus- Genome variation, expression and evolution- Animal and plant molecular cytogenetics and genomics- Chromosome abnormalities and genomic variations in clinical genetics- Applications in preimplantation, pre- and post-natal diagnosis- Applications in the central nervous system, cancer and haematology research- Previously unreported applications of molecular cytogenetic techniques- Development of new techniques or significant enhancements to established techniques. This journal is a source for numerous scientists all over the world, who wish to improve or introduce molecular cytogenetic techniques into their practice.