Effect of silicon or cerium doping on the anti-inflammatory activity of biphasic calcium phosphate scaffolds for bone regeneration.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2022-12-01 Epub Date: 2022-10-12 DOI:10.1007/s40204-022-00206-6
Hyun-Woo Kim, Young-Jin Kim
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引用次数: 2

Abstract

Biphasic calcium phosphate (BCP) bioceramics composed of hydroxyapatite and β-tricalcium phosphate have attracted considerable attention as ideal bone substitutes for reconstructive surgery, orthopedics, and dentistry, owing to their similar chemical composition to bone mineral and biocompatibility. The addition of trace elements to BCP bioceramics, such as magnesium (Mg), cerium (Ce), and silicon (Si), can alter the physicochemical and biological properties of the resulting materials. To improve the anti-inflammatory activity of a pure BCP scaffold, this study developed a simple wet chemical precipitation and gel-casting method to fabricate microporous BCP scaffolds containing Si or Ce. The BCP scaffolds exhibited interconnected microporous structures with uniform micropores and unequiaxed grains. No changes in the phase composition and microstructure of the scaffolds with the Si or Ce doping were observed. Conversely, Si or Ce doping into the BCP crystal lattice influenced the in vitro biological activity of the scaffolds and the bone-forming ability of the cells cultured on the BCP scaffolds. The results of biological activity assays demonstrated that Ce-BCP promoted cell proliferation and osteogenic differentiation more effectively than the other scaffolds. In particular, Ce-BCP significantly suppressed the expression of bone-active cytokines via the anti-inflammatory and anti-oxidative effects. Therefore, Si- or Ce-doped BCP scaffolds can contribute to providing a new generation of bone graft substitutes.

硅或铈掺杂对骨再生双相磷酸钙支架抗炎活性的影响。
由羟基磷灰石和β-磷酸三钙组成的双相磷酸钙(Biphasic calcium phosphate, BCP)生物陶瓷由于具有与骨矿物质相似的化学成分和生物相容性,作为重建外科、骨科和牙科的理想骨替代品而受到广泛关注。在BCP生物陶瓷中添加微量元素,如镁(Mg)、铈(Ce)和硅(Si),可以改变所得材料的物理化学和生物特性。为了提高纯BCP支架的抗炎活性,本研究开发了一种简单的湿化学沉淀法和凝胶浇铸法来制备含Si或Ce的微孔BCP支架。BCP支架的微孔结构相互连接,微孔均匀,颗粒明确。Si或Ce的掺杂对支架的相组成和微观结构没有影响。相反,Si或Ce掺杂到BCP晶格中会影响支架的体外生物活性和BCP支架上培养的细胞成骨能力。生物活性测定结果表明,Ce-BCP比其他支架更能促进细胞增殖和成骨分化。特别是,Ce-BCP通过抗炎和抗氧化作用显著抑制骨活性细胞因子的表达。因此,Si或ce掺杂的BCP支架有助于提供新一代骨移植替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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