[Correlation between the Promoter Methylation Status of TRIM58 and Its mRNA Expression in Acute Myeloid Leukemia].

Zhongguo shi yan xue ye xue za zhi Pub Date : 2022-10-01 DOI:10.19746/j.cnki.issn.1009-2137.2022.05.09

Cheng-Kan DU, Yue Jiang, Ying Lu, Xue Yang

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Abstract

Objective: To analyze the relationship between the promoter methylation status of Tripartite-motif protein 58 (TRIM58) and its mRNA expression level in acute myeloid leukemia (AML), and to explore the expression and regulation of TRIM58 in AML.

Methods: The bisulfite sequencing PCR (BSP) and quantitative real-time PCR (qPCR) technologies were used to detect the promoter methylation status and expression levels of TRIM58 mRNA in primary CD34⁺ and CD34^- AML cells and the AML cell lines KG1a and K562 were determined.

Results: The expression of TRIM58 mRNA in CD34⁺ cells was down-regulated in 10 AML patients, while that in CD34^- cells was down-regulated in 12 cases. Differences in the promoter methylation level of TRIM58 were statistically significant between AML group and normal control group (P<0.05). Additionally, the expression of TRIM58 mRNA was down-regulated in cell lines KG1a and K562, and up-regulated after decitabine treatment.

Conclusion: The down-regulation of mRNA expression of TRIM58 in AML cells may be related to its promoter methylation status.

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[急性髓系白血病中TRIM58启动子甲基化状态与其mRNA表达的相关性]。

目的:分析急性髓性白血病(AML)中Tripartite-motif protein 58 (TRIM58)启动子甲基化状态与其mRNA表达水平的关系，探讨TRIM58在AML中的表达与调控。方法:采用亚硫酸盐测序PCR (BSP)和实时定量PCR (qPCR)技术检测原代CD34+和CD34- AML细胞及AML细胞系KG1a和K562中TRIM58 mRNA启动子甲基化状态和表达水平。结果:10例AML患者CD34+细胞中TRIM58 mRNA表达下调，CD34-细胞中TRIM58 mRNA表达下调12例。AML组与正常对照组之间TRIM58启动子甲基化水平差异有统计学意义(p结论:AML细胞中TRIM58 mRNA表达下调可能与其启动子甲基化状态有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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