[Effect of High-Volume Leukapheresis on Hematological Indexes of Patients with Hyperleukocytic Leukemia].

Zhongguo shi yan xue ye xue za zhi Pub Date : 2022-10-01 DOI:10.19746/j.cnki.issn.1009-2137.2022.05.005

Yong Wu, Yuan-Jun Wu, Hui-Sen Li, Bao-Chan Chen, Yan Liu, Yue-Qin Wu, Si-Yuan Liang

{"title":"[Effect of High-Volume Leukapheresis on Hematological Indexes of Patients with Hyperleukocytic Leukemia].","authors":"Yong Wu, Yuan-Jun Wu, Hui-Sen Li, Bao-Chan Chen, Yan Liu, Yue-Qin Wu, Si-Yuan Liang","doi":"10.19746/j.cnki.issn.1009-2137.2022.05.005","DOIUrl":null,"url":null,"abstract":"OBJECTIVE\nTo improve the collection efficiency of leukapheresis, explore relatively scientific and objective evaluation indicators for collection effect, and observe the effect of high-volume leukapheresis on blood cells and coagulation function.\n\n\nMETHODS\nA total of 158 times of high-volume leukapheresis were performed on 93 patients with hyperleukocytic leukemia by using continuous flow centrifugal blood component separator. 1/5-1/4 of total blood volume of the patients was taken as the target value of leukocyte suspension for single treatment. In addition, the total number of white blood cells (WBCs) subtracted, value of WBCs reduction, rate of WBCs reduction, decrease value of WBCs count, decrease rate of WBCs count, amount of hemoglobin (Hb) lost, value of Hb lost, decreased value of Hb, total number of platelet (PLT) lost, the value of PLT loss, and decrease value of PLT count were used to comprehensively evaluate the collection effect of leukapheresis and influence on Hb level and PLT count of the patients. The prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen (Fib) concentration were detected before and after treatment, and the effect of leukapheresis on coagulation function of the patients was observed.\n\n\nRESULTS\nThe volume of leukocyte suspension collected in a single treatment was 793.01±214.23 ml, the total number of WBCs subtracted was 353.25 (241.99-547.28)×109, the value of WBCs reduction was 86.98 (63.05-143.43)×109/L, the rate of WBCs reduction was 44.24 (28.37-70.48)%, decrease value of WBCs count was 65.73 (37.17-103.97)×109/L, decrease rate of WBCs count was (35.67±23.08)%, the amount of Hb lost was 17.36 (12.12-24.94) g, the value of Hb lost was 4.31 (3.01-6.12) g/L, decreased value of Hb was 4.80 (-1.25-9.33) g/L, total number of PLT lost was 222.79 (67.03-578.31)×109, the value of PLT loss was 54.45 (17.29-139.08)×109/L, and decrease value of PLT count was 26.00 (8.38-62.50)×109/L. Before and after a single treatment, the PT was 14.80 (13.20-16.98) s and 15.20 (13.08-16.90) s (z=-1.520, P>0.05), the aPTT was 35.20 (28.68-39.75) s and 35.40 (28.00-39.75) s (z=-2.058, P<0.05), the TT was 17.50 (16.30-18.80) s and 17.70 (16.70-19.10) s (z=-3.928, P<0.001), and the Fib concentration was 2.87±1.13 g/L and 2.64±1.03 g/L (t=7.151, P<0.001), respectively.\n\n\nCONCLUSION\nHigh-volume leukapheresis can improve the efficiency of leukapheresis while maintaining the relative stability of the patients' circulating blood volume. The degree of influence on the patients' Hb level, PLT count, Fib concentration, and comprehensive coagulation indicators reflecting the patients' intrinsic and cxtrinsic coagulation activity is within the body's compensation range.","PeriodicalId":519535,"journal":{"name":"Zhongguo shi yan xue ye xue za zhi","volume":" ","pages":"1331-1336"},"PeriodicalIF":0.0000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo shi yan xue ye xue za zhi","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2022.05.005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

OBJECTIVE To improve the collection efficiency of leukapheresis, explore relatively scientific and objective evaluation indicators for collection effect, and observe the effect of high-volume leukapheresis on blood cells and coagulation function. METHODS A total of 158 times of high-volume leukapheresis were performed on 93 patients with hyperleukocytic leukemia by using continuous flow centrifugal blood component separator. 1/5-1/4 of total blood volume of the patients was taken as the target value of leukocyte suspension for single treatment. In addition, the total number of white blood cells (WBCs) subtracted, value of WBCs reduction, rate of WBCs reduction, decrease value of WBCs count, decrease rate of WBCs count, amount of hemoglobin (Hb) lost, value of Hb lost, decreased value of Hb, total number of platelet (PLT) lost, the value of PLT loss, and decrease value of PLT count were used to comprehensively evaluate the collection effect of leukapheresis and influence on Hb level and PLT count of the patients. The prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen (Fib) concentration were detected before and after treatment, and the effect of leukapheresis on coagulation function of the patients was observed. RESULTS The volume of leukocyte suspension collected in a single treatment was 793.01±214.23 ml, the total number of WBCs subtracted was 353.25 (241.99-547.28)×109, the value of WBCs reduction was 86.98 (63.05-143.43)×109/L, the rate of WBCs reduction was 44.24 (28.37-70.48)%, decrease value of WBCs count was 65.73 (37.17-103.97)×109/L, decrease rate of WBCs count was (35.67±23.08)%, the amount of Hb lost was 17.36 (12.12-24.94) g, the value of Hb lost was 4.31 (3.01-6.12) g/L, decreased value of Hb was 4.80 (-1.25-9.33) g/L, total number of PLT lost was 222.79 (67.03-578.31)×109, the value of PLT loss was 54.45 (17.29-139.08)×109/L, and decrease value of PLT count was 26.00 (8.38-62.50)×109/L. Before and after a single treatment, the PT was 14.80 (13.20-16.98) s and 15.20 (13.08-16.90) s (z=-1.520, P>0.05), the aPTT was 35.20 (28.68-39.75) s and 35.40 (28.00-39.75) s (z=-2.058, P<0.05), the TT was 17.50 (16.30-18.80) s and 17.70 (16.70-19.10) s (z=-3.928, P<0.001), and the Fib concentration was 2.87±1.13 g/L and 2.64±1.03 g/L (t=7.151, P<0.001), respectively. CONCLUSION High-volume leukapheresis can improve the efficiency of leukapheresis while maintaining the relative stability of the patients' circulating blood volume. The degree of influence on the patients' Hb level, PLT count, Fib concentration, and comprehensive coagulation indicators reflecting the patients' intrinsic and cxtrinsic coagulation activity is within the body's compensation range.

查看原文本刊更多论文

[高容量白细胞分离对高白细胞白血病患者血液学指标的影响]。

目的:提高白细胞采集效率，探索相对科学客观的采集效果评价指标，观察大容量白细胞采集对血细胞及凝血功能的影响。方法:对93例高白细胞白血病患者采用连续流式离心式血液成分分离器进行158次大容量白细胞分离。单次治疗以患者总血容量的1/5 ~ 1/4为白细胞悬浮液目标值。此外,白细胞的总数(白细胞)减去,白细胞减少,白细胞减少,降低白细胞计数的价值,降低白细胞计数,血红蛋白(Hb)丢失,Hb失去的价值,减少乙肝的价值,血小板(PLT)失去的总数、PLT的价值损失,减少的价值PLT数被用来全面评估leukapheresis的集合效应和影响Hb水平,PLT计数的患者。检测治疗前后凝血酶原时间(PT)、活化部分凝血活酶时间(aPTT)、凝血酶时间(TT)、纤维蛋白原(Fib)浓度，并观察白细胞分离对患者凝血功能的影响。结果:白细胞的数量收集悬浮在一个治疗793.01±214.23毫升,白细胞总数减去353.25(241.99 - -547.28)×109,白细胞减少的价值是86.98(63.05 - -143.43)×109 / L,白细胞减少的速度是44.24(28.37 - -70.48)%,降低白细胞计数的价值为65.73(37.17 - -103.97)×109 / L,降低白细胞计数率(35.67±23.08)%,Hb丢失的数量是17.36 (12.12 - -24.94)g, Hb失去的价值为4.31 (3.01 - -6.12)g / L,Hb降低值为4.80 (-1.25-9.33)g/L, PLT丢失总数为222.79 (67.03-578.31)×109, PLT丢失值为54.45 (17.29-139.08)×109/L, PLT计数减少值为26.00 (8.38-62.50)×109/L。单次治疗前后PT分别为14.80 (13.20 ~ 16.98)s和15.20 (13.08 ~ 16.90)s (z=-1.520, P>0.05)， aPTT分别为35.20 (28.68 ~ 39.75)s和35.40 (28.00 ~ 39.75)s (z=-2.058, P>0.05)。结论:大容量白细胞采集可在保持患者循环血容量相对稳定的同时提高白细胞采集效率。对患者Hb水平、PLT计数、Fib浓度以及反映患者内外凝血活性的综合凝血指标的影响程度在机体可补偿范围内。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Zhongguo shi yan xue ye xue za zhi

自引率

0.00%

发文量