The regulation of proliferation and apoptosis in hepatocellular carcinoma via insulin-like growth factor 1 receptor

IF 1.6 4区医学 Q4 CELL BIOLOGY

Growth Hormone & Igf Research Pub Date : 2022-10-01 DOI:10.1016/j.ghir.2022.101499

Guanjun Xu, Jiesheng Chu, Yu Shi, Longzhang Huang, Jingzhong Fu

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引用次数: 0

Abstract

Objectives

Insulin-like growth factor 1 receptor (IGF-1R) is a transmembrane tyrosine kinase receptor of the insulin receptor family. Its expression is consistently increased in hepatocellular carcinoma (HCC) tissue, and it participates in hepatic carcinogenesis. Targeting IGF-1R may be a potential therapeutic approach against hepatocellular carcinoma. This study therefore aimed to explore the effect of IGF-1R on hepatocellular carcinoma cells.

Methods

IGF-1R silencing cell lines were established by small-interfering RNAs in hepatocellular carcinoma cell line SMMC7721, after which the proliferation, invasion, and apoptosis of SMMC7721 was evaluated. The activation of the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signaling pathway and the expression of bone morphogenetic protein (BMP)-2 and BMP-7 were measured using Western blot analysis.

Results

The results indicated that the knockdown of IGF-1R can inhibit the proliferation and invasion of HCC and promote the apoptosis of SMMC7721 by inhibiting the PI3K/AKT signaling pathway. Furthermore, depletion of IGF-1R was found to suppress the expression of BMP-2 and BMP-7.

Conclusions

The findings suggest that IGF-1R plays an important role in the progression of HCC. Therefore, IGF-1R is a potential target for the treatment of HCC in clinic.

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胰岛素样生长因子1受体对肝癌细胞增殖和凋亡的调控

目的胰岛素样生长因子1受体(IGF-1R)是胰岛素受体家族中的一种跨膜酪氨酸激酶受体。它在肝细胞癌(HCC)组织中的表达持续增加，参与肝癌的发生。靶向IGF-1R可能是一种潜在的治疗肝细胞癌的方法。因此，本研究旨在探讨IGF-1R对肝癌细胞的影响。方法利用小干扰rna在肝癌细胞系SMMC7721中建立sigf - 1r沉默细胞株，观察其增殖、侵袭和凋亡情况。Western blot检测各组大鼠PI3K / AKT信号通路的激活及骨形态发生蛋白(BMP)-2和BMP-7的表达。结果表明，IGF-1R的下调可通过抑制PI3K/AKT信号通路抑制肝癌细胞的增殖和侵袭，促进SMMC7721细胞的凋亡。此外，IGF-1R的缺失被发现抑制BMP-2和BMP-7的表达。结论IGF-1R在HCC的发生发展中起重要作用。因此，IGF-1R是临床上治疗HCC的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth Hormone & Igf Research 医学-内分泌学与代谢

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

57 days

期刊介绍： Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.