Bruna Dias Espindola, Fagner D'ambroso Fernandes, Isac Junior Roman, Gisele Vaz Aguirre Samoel, Roberto Antônio Delgado Barcelos, Alisson Rodrigues Döhler, Sônia de Ávila Botton, Fernanda Silveira Flores Vogel, Luis Antonio Sangioni
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引用次数: 0
Abstract
The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destined for human consumption, including: salami, sausage, black pudding, heart, tongue, brain, and rib muscle, were submitted to PCR to detect DNA for each agent. The frequency of anti-Sarcocystis spp. antibodies was 36.9% (31/84), with titers ranging from 32 to 1024, and 25% (21/84) for anti-T. gondii antibodies, with titers ranging from 64 to 2048. Sarcocystis spp. and T. gondii DNA were detected in 67.9% (36/53) and 13.2% (7/53) of samples, respectively. The presence of antibodies and the detection of DNA from Sarcocystis spp., and T. gondii suggests that the pigs were infected and may serve as an important reservoir for both parasites. The infection by these protozoa in the swine population is relevant to public health due to their zoonotic potential.
研究了巴西RS Santa Maria饲养的猪中肌囊虫和刚地弓形虫的血清流行率。采用间接免疫荧光法（IFA）对来自31个猪场的84头猪的血清样本进行了两种病原体的检测。此外，53份供人类食用的猪肉香肠和组织样本，包括：意大利腊肠、香肠、黑布丁、心脏、舌头、大脑和肋骨肌，被提交给PCR检测每种病原体的DNA。抗体阳性率为36.9%(31/84)，滴度为32 ~ 1024；抗体阳性率为25%（21/84）。弓形虫抗体，滴度从64到2048不等。67.9%（36/53）和13.2%（7/53）的样本检出肉囊菌和弓形虫DNA。抗体的存在和刚地弓形虫和肉囊虫DNA的检测表明，这些猪被感染，可能是这两种寄生虫的重要宿主。这些原生动物在猪群中的感染与公共卫生有关，因为它们具有人畜共患的潜力。
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