Encapsulation of Phaseolus lunatus Protein Hydrolysate with Angiotensin-Converting Enzyme Inhibitory Activity.

ISRN biotechnology Pub Date : 2013-09-24 eCollection Date: 2013-01-01 DOI:10.5402/2013/341974

Jorge Carlos Ruiz Ruiz, Maira Rubí Segura Campos, David Abram Betancur Ancona, Luis Antonio Chel Guerrero

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引用次数: 17

Abstract

The objective of recent research has been to seek alternative therapeutic treatments; for this reason, the use of protein hydrolysates from diverse sources has been studied. A way to guarantee that these treatments reach the site of action is through protection with covers, such as microcapsules. Therefore, proteins from the legume Phaseolus lunatus were hydrolyzed and encapsulated with a blend of Delonix regia carboxymethylated gum/sodium alginate (50 : 50 w/w). Hydrolysis release conditions in a simulated gastrointestinal system were obtained using CaCl2 concentrations as the main factor, indicating that lower CaCl2 concentrations lead to an increased hydrolysis release. Beads obtained with 1.0 mM of CaCl2 exhibited a better hydrolysate release rate under intestinal simulated conditions and the proteins maintained an IC50 of 2.9 mg/mL. Capsules obtained with the blend of Delonix regia carboxymethylated gum/sodium alginate would be used for the controlled delivery of hydrolysates with potential use as nutraceutical or therapeutic agents.

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具有血管紧张素转换酶抑制活性的菜豆蛋白水解物包封。

最近研究的目的是寻求替代治疗方法;出于这个原因，人们研究了不同来源的蛋白质水解物的使用。保证这些药物到达作用部位的一种方法是使用保护膜，例如微胶囊。因此，豆科植物Phaseolus lunatus中的蛋白质被Delonix regia羧甲基化胶/海藻酸钠(50:50 w/w)的混合物水解并包封。以CaCl2浓度为主要因素获得了模拟胃肠道系统中的水解释放条件，表明较低的CaCl2浓度导致水解释放增加。在肠道模拟条件下，1.0 mM CaCl2获得的微球具有更好的水解产物释放率，蛋白质的IC50维持在2.9 mg/mL。由德龙尼羧甲基化胶/海藻酸钠混合制成的胶囊可用于控制水解产物的递送，有可能用作营养品或治疗剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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ISRN biotechnology

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