[The study of daughter rediae Echinostoma caproni (Trematoda) in vitro cultivation].

Abstract

Methods of in vitro cultivation were used to examine the feeding and reproductive behaviors of daughter rediae of Echinostoma caproni. It was noted that under conditions of in vitro cultivation, rediae fed on tissues of the mollusc Biomphalaria glabrata, on rediae and cercariae of E. daikenaensis and E. congoensis, and on sporocysts and cercariae of Schistosoma mansoni. No cases of cannibalism of daughter rediae E. caproni by their offspring rediae were observed, although they could feed on their own cercariae. When kept in mediae containing (B. glabrata embryonic) Bge cells, rediae E. caproni gradually turned to feeding on these cells and stayed away other objects. Under conditions of in vitro cultivation, daughter rediae E. caproni were capable of forming redial and cercarial embryos. However, no cases of return from producing of cercariae to producing of rediae were observed. These in vitro data confirm the results of previous studies of this species's parthenithae performed in vivo (ATaeB and dp., 2007).