Aging Research Using Mouse Models

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-06-01 DOI:10.1002/9780470942390.mo140195

Cheryl L. Ackert-Bicknell, Laura C. Anderson, Susan Sheehan, Warren G. Hill, Bo Chang, Gary A. Churchill, Elissa J. Chesler, Ron Korstanje, Luanne L. Peters

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引用次数: 85

Abstract

Despite the dramatic increase in human lifespan over the past century, there remains pronounced variability in “health-span,” or the period of time in which one is generally healthy and free of disease. Much of the variability in health-span and lifespan is thought to be genetic in origin. Understanding the genetic mechanisms of aging and identifying ways to boost longevity is a primary goal in aging research. Here, we describe a pipeline of phenotypic assays for assessing mouse models of aging. This pipeline includes behavior/cognition testing, body composition analysis, and tests of kidney function, hematopoiesis, and immune function, as well as physical parameters. We also describe study design methods for assessing lifespan and health-span, and other important considerations when conducting aging research in the laboratory mouse. The tools and assays provided can assist researchers with understanding the correlative relationships between age-associated phenotypes and, ultimately, the role of specific genes in the aging process. © 2015 by John Wiley & Sons, Inc.

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使用小鼠模型进行衰老研究

尽管在过去的一个世纪里，人类的寿命急剧增加，但“健康寿命”(即一个人总体上健康且没有疾病的时间段)仍然存在明显的差异。健康寿命和寿命的许多差异被认为是源于基因。了解衰老的遗传机制并确定延长寿命的方法是衰老研究的主要目标。在这里，我们描述了一个管道表型分析评估小鼠模型的衰老。该管道包括行为/认知测试、身体成分分析、肾功能、造血、免疫功能以及身体参数的测试。我们还描述了评估寿命和健康寿命的研究设计方法，以及在实验室小鼠中进行衰老研究时的其他重要考虑因素。提供的工具和分析可以帮助研究人员了解年龄相关表型之间的相关关系，并最终了解特定基因在衰老过程中的作用。©2015 by John Wiley &儿子,Inc。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.