Regulation of neutrophil infiltration into peritoneal cavity by laparoscopic gastrectomy.

Hepato-gastroenterology Pub Date : 2015-03-01

Mami Yoshii, Hiroaki Tanaka, Masaichi Ohira, Kazuya Muguruma, Tomohiro Lee, Katsunobu Sakurai, Naoshi Kubo, Kiyoshi Maeda, Kosei Hirakawa

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引用次数: 0

Abstract

Background/aims: Laparoscopic surgery is a minimally invasive operation developed for treating gastrointestinal malignancies. We aimed to characterize the differences in the intra-abdominal environment following open and laparoscopic surgeries.

Methodology: We investigated data of 48 patients who underwent gastrectomy between 2010 and 2012. We analyzed the mRNA expression of chemokines, indoleamine 2, 3-dioxygenase (IDO), and so on in peritoneal lavage fluid with real-time RT-PCR. We also determined the leukocyte population and calculated the granulocyte/lymphocyte (G/L) ratio in peritoneal lavage fluid using flow cytometry.

Results: CCL3 mRNA was significantly upregulated, whereas IDO mRNA was significantly downregulated, in the open group compared to the laparoscopic surgery group. Flow cytometry revealed that the G/L ratio was significantly higher in the open group.

Conclusions: We suggest that the production of chemokines and neutrophil infiltration into the abdominal cavity may be suppressed in the laparoscopic surgery. Thus, laparoscopic surgery may be beneficial in preserving local immunity.

本刊更多论文

腹腔镜胃切除术对中性粒细胞腹腔浸润的调节。

背景/目的:腹腔镜手术是一种用于治疗胃肠道恶性肿瘤的微创手术。我们的目的是描述在开放和腹腔镜手术后腹内环境的差异。方法:我们调查了2010年至2012年间接受胃切除术的48例患者的资料。采用实时RT-PCR方法分析腹腔灌洗液中趋化因子、吲哚胺2,3 -双加氧酶(IDO)等mRNA的表达。我们还用流式细胞术测定了腹膜灌洗液中的白细胞群，并计算了粒细胞/淋巴细胞(G/L)的比值。结果:与腹腔镜手术组相比，开放组CCL3 mRNA显著上调，IDO mRNA显著下调。流式细胞术显示，开放组的G/L比值明显升高。结论:腹腔镜手术可能抑制了趋化因子的产生和中性粒细胞的腹腔浸润。因此，腹腔镜手术可能有利于保持局部免疫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hepato-gastroenterology 医学-外科

自引率

0.00%

发文量

审稿时长

1.9 months

期刊介绍： Hepato-Gastroenterology has been discontinued as of 2015. Extremely limited quantities of back issues in print available for sale.