Impact of GLP-1 analogue on oxidative damage and hepatic regeneration in experimental 70% hepatectomy model.

Hepato-gastroenterology Pub Date : 2015-03-01

Muharrem Battal, Bulent Çitgez, Abdulcabbar Kartal, Ahu Kemik, Pinar Yildirim, Yasar Ozdenkaya, Ahmet Yilmaz, Oguzhan Karatepe

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引用次数: 0

Abstract

Background/aims: The purpose of our study is researching into impact of glucagon like peptide 1 (GLP 1) analogue on liver regeneration after major hepatectomy.

Methodology: 24 wistar albino rats were consecutively divided into 3 groups. Group 1: Control (sham) group day 14 (n = 8), Group 2: Liver resection group day 14 (n = 8); 70% Liver resection was performed, Group 3: Study group day 14 (n = 8); Subsequent to performing 70% liver resection, GLP-1 analogue was administered 2 times a day. (10 μgr/70 kg x 2 times). After 14 day, rats were sacrificed. Oxi- dative stress and antioxidant enzymes and mitochon- drial permeability transition, cytochrome-c, Bax, Bcl- 2, caspase-3, caspase-8 and caspase-3 activity were examined.

Results: 70% Liver resection induced oxi- dative stress of liver tissue was ameliorated by GLP-1 induction. Administration of GLP increased Bcl-2 ex- pression. Decreased expression of cytochrome-c was accompanied by a decrease caspase-3, caspase-8, and Bax expression and caspase-3 activity.

Conclusions: Glp 1 induction plays a regenerative role in the major hepatectomy. This effect is dependent on modulation of the antiapoptotic and antioxidative pathways by GLP 1 expression.

本刊更多论文

GLP-1类似物对实验性70%肝切除模型氧化损伤和肝再生的影响。

背景/目的:本研究旨在探讨胰高血糖素样肽1 (glp1)类似物对肝切除术后肝再生的影响。方法:将24只wistar白化大鼠连续分为3组。组1:对照组(假手术)第14天(n = 8)，组2:肝切除组第14天(n = 8);70%肝切除，第3组:研究组第14天(n = 8);切除70%肝脏后，每天给予GLP-1类似物2次。(10 μgr/70 kg × 2次)。14天后，处死大鼠。检测氧化应激、抗氧化酶、线粒体通透性转变、细胞色素c、Bax、Bcl- 2、caspase-3、caspase-8、caspase-3活性。结果:GLP-1诱导能改善70%肝切除术后肝组织氧化应激。GLP可增加Bcl-2的表达。细胞色素c表达降低，caspase-3、caspase-8、Bax表达降低，caspase-3活性降低。结论:glp1诱导在大肝切除术中具有再生作用。这种作用依赖于glp1表达对抗凋亡和抗氧化途径的调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hepato-gastroenterology 医学-外科

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0.00%

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审稿时长

1.9 months

期刊介绍： Hepato-Gastroenterology has been discontinued as of 2015. Extremely limited quantities of back issues in print available for sale.