Alkaline and ultrasonic dissolution of biological materials for trace silicon determination.

IF 1.5 4区 工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC
Robert D Viveros, Alexander Liberman, William C Trogler, Andrew C Kummel
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引用次数: 0

Abstract

A simple method for trace elemental determination in biological tissue has been developed. Novel nanomaterials with biomedical applications necessitate the determination of the in vivo fate of the materials to understand their toxicological profile. Hollow iron-doped calcined silica nanoshells have been used as a model to demonstrate that potassium hydroxide and bath sonication at 50 °C can extract elements from alkaline-soluble nanomaterials. After alkali digestion, nitric acid is used to adjust the pH into a suitable range for analysis using techniques such as inductively coupled plasma optical emission spectrometry which require neutral or acidic analytes. In chicken liver phantoms injected with the nanoshells, 96% of the expected silicon concentration was detected. This value was in good agreement with the 94% detection efficiency of nanoshells dissolved in aqueous solution as a control for potential sample matrix interference. Nanoshell detection was further confirmed in a mouse 24 h after intravenous administration; the measured silica above baseline was 35 times greater or more than the standard deviations of the measurements. This method provides a simple and accurate means to quantify alkaline-soluble nanomaterials in biological tissue.

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碱性和超声波溶解生物材料以测定痕量硅。
已开发出一种测定生物组织中痕量元素的简单方法。具有生物医学应用价值的新型纳米材料需要测定其体内转归,以了解其毒理学特征。以掺铁的中空煅烧二氧化硅纳米壳为模型,证明氢氧化钾和 50 ℃水浴超声处理可从碱溶性纳米材料中提取元素。碱消化后,使用硝酸将 pH 值调节到合适的范围,以便使用电感耦合等离子体光发射光谱等需要中性或酸性分析物的技术进行分析。在注入纳米壳的鸡肝模型中,检测到了 96% 的预期硅浓度。这一数值与溶解在水溶液中的纳米壳 94% 的检测效率非常吻合。小鼠静脉注射纳米壳 24 小时后,纳米壳的检测结果得到了进一步证实;测得的硅浓度比基线高出 35 倍,甚至超过了测量值的标准偏差。该方法为量化生物组织中的碱溶性纳米材料提供了一种简单而准确的方法。
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来源期刊
CiteScore
2.70
自引率
0.00%
发文量
146
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