Role of Pex11p in Lipid Homeostasis in Yarrowia lipolytica.

Eukaryotic Cell Pub Date : 2015-05-01 Epub Date: 2015-03-27 DOI:10.1128/EC.00051-15
Rémi Dulermo, Thierry Dulermo, Heber Gamboa-Meléndez, France Thevenieau, Jean-Marc Nicaud
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引用次数: 16

Abstract

Peroxisomes are essential organelles in the cells of most eukaryotes, from yeasts to mammals. Their role in β-oxidation is particularly essential in yeasts; for example, in Saccharomyces cerevisiae, fatty acid oxidation takes place solely in peroxisomes. In this species, peroxisome biogenesis occurs when lipids are present in the culture medium, and it involves the Pex11p protein family: ScPex11p, ScPex25p, ScPex27p, and ScPex34p. Yarrowia lipolytica has three Pex11p homologues, which are YALI0C04092p (YlPex11p), YALI0C04565p (YlPex11C), and YALI0D25498p (Pex11/25p). We found that these genes are regulated by oleic acid, and as has been observed in other organisms, YlPEX11 deletion generated giant peroxisomes when mutant yeast were grown in oleic acid medium. Moreover, ΔYlpex11 was unable to grow on fatty acid medium and showed extreme dose-dependent sensitivity to oleic acid. Indeed, when the strain was grown in minimum medium with 0.5% glucose and 3% oleic acid, lipid body lysis and cell death were observed. Cell death and lipid body lysis may be partially explained by an imbalance in the expression of the genes involved in lipid storage, namely, DGA1, DGA2, and LRO1, as well as that of TGL4, which is involved in lipid remobilization. TGL4 deletion and DGA2 overexpression resulted in decreased oleic acid sensitivity and delayed cell death of ΔYlpex11, which probably stemmed from the release of free fatty acids into the cytoplasm. All these results show that YlPex11p plays an important role in lipid homeostasis in Y. lipolytica.

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Pex11p在溶脂耶氏菌脂质稳态中的作用。
过氧化物酶体是大多数真核生物(从酵母到哺乳动物)细胞中必不可少的细胞器。它们在β氧化中的作用在酵母中尤为重要;例如,在酿酒酵母中,脂肪酸氧化只发生在过氧化物酶体中。在这个物种中,当培养基中存在脂质时,过氧化物酶体就会发生生物发生,它涉及到Pex11p蛋白家族:ScPex11p、ScPex25p、ScPex27p和ScPex34p。脂溶耶氏菌有三个Pex11p同源物,分别是YALI0C04092p (YlPex11p)、YALI0C04565p (YlPex11C)和YALI0D25498p (Pex11/25p)。我们发现这些基因受到油酸的调控,并且正如在其他生物中观察到的那样,当突变酵母在油酸培养基中生长时,YlPEX11缺失产生了巨大的过氧化物酶体。此外,ΔYlpex11不能在脂肪酸培养基上生长,对油酸表现出极强的剂量依赖性敏感性。事实上,当菌株在含有0.5%葡萄糖和3%油酸的最低培养基中生长时,观察到脂体裂解和细胞死亡。参与脂质储存的基因DGA1、DGA2和LRO1以及参与脂质再动员的TGL4的表达失衡可能是细胞死亡和脂质体裂解的部分原因。TGL4缺失和DGA2过表达导致ΔYlpex11油酸敏感性降低和细胞死亡延迟,这可能与游离脂肪酸释放到细胞质中有关。这些结果表明,YlPex11p在脂肪瘤的脂质稳态中起重要作用。
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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
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审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
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