Platonin preserves blood–brain barrier integrity in septic rats

Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists Pub Date : 2015-03-01 DOI:10.1016/j.aat.2015.02.001

Chia-Tse Yeh , Ming-Chang Kao , Cay-Huyen Chen , Chun-Jen Huang

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引用次数: 13

Abstract

Objectives

Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood–brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats.

Methods

A total of 72 adult male rats (200–250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring.

Results

Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA)—markers of systemic inflammation and oxidation—and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels—markers of BBB integrity—in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023).

Conclusion

Platonin could attenuate sepsis-induced BBB integrity loss in rats.

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铂蛋白可保持脓毒症大鼠血脑屏障的完整性

目的柏拉图蛋白具有较强的抗炎和抗氧化能力。由于全身炎症和氧化应激在介导败血症诱导的血脑屏障(BBB)完整性丧失中至关重要，本研究阐明了铂蛋白在保护败血症大鼠血脑屏障完整性方面的作用。方法选取成年雄性大鼠72只(200 ~ 250 g)，随机分为盲肠结扎穿刺组(CLP)、CLP +铂宁组(CLP +铂宁组)、假手术组(每组18只)和假手术组(每组18只)。24小时监测存活大鼠全身炎症、氧化水平和血脑屏障完整性。结果CLP大鼠血浆白细胞介素-6 (IL-6)和丙二醛(MDA)水平(全身炎症和氧化标志物)、脑Evans蓝染色分级、血脑屏障对Evans蓝染料的通透性和脑水肿水平(血脑屏障完整性标志物)均显著高于假手术大鼠(p <0.001)。相比之下，血浆IL-6水平(p <0.001)和MDA (p <CLP +铂肽组大鼠的Evans蓝染色分级(p = 0.015)、血脑屏障对Evans蓝染料的通透性(p = 0.043)和脑水肿水平(p = 0.034)均显著低于CLP组大鼠。实验数据进一步显示，CLP组大鼠血脑屏障主要结构成分紧密连接蛋白cludin -5的浓度显著低于CLP + platonin组(p = 0.023)。结论铂宁可减轻脓毒症所致大鼠血脑屏障完整性丧失。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists

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