Effect of Diffusion on Discoloration of Congo Red by Alginate Entrapped Turnip (Brassica rapa) Peroxidase.

Q2 Biochemistry, Genetics and Molecular Biology

Enzyme Research Pub Date : 2015-01-01 Epub Date: 2015-02-05 DOI:10.1155/2015/575618

Afaf Ahmedi, Mahmoud Abouseoud, Amrane Abdeltif, Couvert Annabelle

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引用次数: 11

Abstract

Enzymatic discoloration of the diazo dye, Congo red (CR), by immobilized plant peroxidase from turnip "Brassica rapa" is investigated. Partially purified turnip peroxidase (TP) was immobilized by entrapment in spherical particles of calcium alginate and was assayed for the discoloration of aqueous CR solution. Experimental data revealed that pH, reaction time, temperature, colorant, and H2O2 concentration play a significant role in dye degradation. Maximum CR removal was found at pH 2.0, constant temperature of 40°C in the presence of 10 mM H2O2, and 180 mg/L of CR. More than 94% of CR was removed by alginate immobilized TP after 1 h of incubation in a batch process under optimal conditions. About 74% removal efficiency was retained after four recycles. Diffusional limitations in alginate beads such as effectiveness factor η, Thiele modulus Φ, and effective diffusion coefficients (D e ) of Congo red were predicted assuming a first-order biodegradation kinetic. Results showed that intraparticle diffusion resistance has a significant effect on the CR biodegradation rate.

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海藻酸盐包埋芜菁过氧化物酶扩散对刚果红变色的影响。

研究了固定化芜菁植物过氧化物酶对重氮染料刚果红(CR)的酶促变色作用。用海藻酸钙球粒包埋法固定化部分纯化的萝卜过氧化物酶(TP)，并测定其在CR水溶液中的变色情况。实验数据表明，pH、反应时间、温度、着色剂和H2O2浓度对染料降解有显著影响。在pH为2.0、温度为40℃、H2O2浓度为10 mM、CR浓度为180 mg/L的条件下，藻酸盐固定化TP的CR去除率达到94%以上。4次循环后，去除率仍保持在74%左右。在一级生物降解动力学条件下，预测了藻酸盐微球的扩散限制，如有效因子η、Thiele模量Φ和刚果红的有效扩散系数(D e)。结果表明，颗粒内扩散阻力对CR的生物降解率有显著影响。

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来源期刊

Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

4.60

自引率

0.00%

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