[Change in the concentration of intracellular ATP during adhesion of Rhodococcus ruber gt1 and pseudomonas fluorescens C2 cells on carbon supports].

Abstract

The effect of immobilization of nitrile-utilizing bacteria Rhodococcus ruber gt1 and Pseudomonasfluorescens C2 by adhesion on carbon supports on the content of the intracellular ATP immediately after adsorption and after 2 h and 24-48 h after transfer of the adhered cells into a fresh nutrient medium was studied. Adhesion was shown to lead to a decreased concentration ofATP in a cell by one order of magnitude or more in the first hours in a fresh nutrient medium that'can be attributed to energetic consumption upon the initiation of biofilm formation. A gradual rise in the quantity of ATP, which was calculated per 1 mg of adsorbed cells, was reported to take place in daily and two-daily biofilms, which confirms the cells remain viable.