Glycosaminoglycans in the human cornea: age-related changes.

Ophthalmology and eye diseases Pub Date : 2015-01-27 eCollection Date: 2015-01-01 DOI:10.4137/OED.S17204

Elena Pacella, Fernanda Pacella, Giulio De Paolis, Francesca Romana Parisella, Paolo Turchetti, Giulia Anello, Carlo Cavallotti

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Abstract

Aim: To investigate possible age-related changes in glycosaminoglycans (GAGs) in the human cornea. The substances today called GAGs were previously referred to as mucopolysaccharides.

Methods: Samples of human cornea were taken from 12 younger (age 21 ± 1.2) and 12 older (age 72 ± 1.6) male subjects. Samples were weighed, homogenized, and used for biochemical and molecular analyses. All the quantitative results were statistically analyzed.

Results: The human cornea appears to undergo age-related changes, as evidenced by our biochemical and molecular results. The total GAG and hyaluronic acid counts were significantly higher in the younger subjects than in the older subjects. The sulfated heavy GAGs, such as chondroitin, dermatan, keratan, and heparan sulfate, were lower in the younger subjects than in the older subjects.

Discussion: GAGs of the human cornea undergo numerous age-related changes. Their quantity is significantly altered in the elderly in comparison with younger subjects. GAGs play an important role in age-related diseases of the human cornea.

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人类角膜中的糖胺聚糖：与年龄有关的变化。

目的：研究人类角膜中与年龄有关的糖胺聚糖（GAGs）的可能变化。如今被称为 GAGs 的物质以前被称为粘多糖：从 12 名年龄较小（21 ± 1.2 岁）和 12 名年龄较大（72 ± 1.6 岁）的男性受试者身上采集人类角膜样本。样本经称重、均质后用于生化和分子分析。所有定量结果均进行了统计分析：结果：我们的生化和分子分析结果表明，人类角膜似乎发生了与年龄相关的变化。年轻受试者的总 GAG 和透明质酸数量明显高于老年受试者。硫酸化的重型 GAGs，如软骨素、真皮鞣质、角朊和硫酸天门冬酰胺在年轻受试者中的含量低于老年受试者：讨论：人类角膜的 GAGs 会发生许多与年龄相关的变化。讨论：人类角膜上的 GAGs 会发生许多与年龄相关的变化，与年轻人相比，老年人角膜上的 GAGs 数量会发生明显变化。GAGs 在人类角膜与年龄有关的疾病中发挥着重要作用。

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Ophthalmology and eye diseases

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8 weeks