Dynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.

Eukaryotic Cell Pub Date : 2015-03-01 Epub Date: 2015-01-09 DOI:10.1128/EC.00141-14
Zhiwei Zhu, Yunfeng Ding, Zhiwei Gong, Li Yang, Sufang Zhang, Congyan Zhang, Xinping Lin, Hongwei Shen, Hanfa Zou, Zhensheng Xie, Fuquan Yang, Xudong Zhao, Pingsheng Liu, Zongbao K Zhao
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引用次数: 70

Abstract

Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. Manipulating LD formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. However, key factors regulating LD dynamics remain poorly characterized. Here we purified the LDs and identified LD-associated proteins from cells of the lipid-producing yeast Rhodosporidium toruloides cultured under nutrient-rich, nitrogen-limited, and phosphorus-limited conditions. The LD proteome consisted of 226 proteins, many of which are involved in lipid metabolism and LD formation and evolution. Further analysis of our previous comparative transcriptome and proteome data sets indicated that the transcription level of 85 genes and protein abundance of 77 proteins changed under nutrient-limited conditions. Such changes were highly relevant to lipid accumulation and partially confirmed by reverse transcription-quantitative PCR. We demonstrated that the major LD structure protein Ldp1 is an LD marker protein being upregulated in lipid-rich cells. When overexpressed in Saccharomyces cerevisiae, Ldp1 localized on the LD surface and facilitated giant LD formation, suggesting that Ldp1 plays an important role in controlling LD dynamics. Our results significantly advance the understanding of the molecular basis of lipid overproduction and storage in oleaginous yeasts and will be valuable for the development of superior lipid producers.

Abstract Image

Abstract Image

产油酵母环形红孢子虫脂滴蛋白质组动力学。
脂滴(ld)是一种普遍存在的细胞器,作为中性脂质储存库和脂质代谢中心。操纵产油物种中LD的形成、进化和动员可能导致脂肪酸衍生生物燃料和化学品的生产。然而,调节LD动力学的关键因素仍然缺乏表征。本研究从富营养、限氮和限磷条件下培养的产脂酵母toruloides Rhodosporidium细胞中纯化了ld并鉴定了ld相关蛋白。LD蛋白质组由226个蛋白组成,其中许多蛋白参与脂质代谢和LD的形成和演化。进一步分析我们之前的比较转录组和蛋白质组数据集表明,85个基因的转录水平和77个蛋白质的蛋白质丰度在营养限制条件下发生了变化。这种变化与脂质积累高度相关,并通过逆转录-定量PCR得到部分证实。我们证明了主要的LD结构蛋白Ldp1是一个LD标记蛋白,在富含脂质的细胞中上调。当在酿酒酵母中过表达时,Ldp1定位于LD表面,促进了巨大LD的形成,这表明Ldp1在控制LD动力学中起重要作用。我们的研究结果极大地促进了对产油酵母中油脂过量生产和储存的分子基础的理解,并将为开发优质的油脂生产者提供价值。
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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
自引率
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0
审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
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