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{"title":"Quantification of Th1 and Th17 Cells with Intracellular Staining Following PMA/Ionomycin Stimulation","authors":"Fridrik Karlsson, Mina Hassan-Zahraee","doi":"10.1002/0471142956.cy0635s71","DOIUrl":null,"url":null,"abstract":"<p>Cytokine-producing cells are at the center of the adaptive immune responses, and quantifying these cells is an important aspect to build understanding of the immune response. In particular, Th1 and Th17 cells have been implicated in the pathogenesis of such diseases as inflammatory bowel disease, rheumatoid arthritis, and multiple sclerosis. Quantification of Th1 and Th17 cells can provide important information in research of these diseases and other Th1- and Th17-mediated immune disorders. In vitro stimulation of cells followed by surface and intracellular staining, presented here, has the advantage of detecting the cytokines directly instead of relying exclusively on surrogate surface markers which, although showing enrichment for the effector T cells, are not specific markers for the cytokine-producing cells. © 2015 by John Wiley & Sons, Inc.</p>","PeriodicalId":11020,"journal":{"name":"Current Protocols in Cytometry","volume":"71 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2015-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/0471142956.cy0635s71","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cytometry","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/0471142956.cy0635s71","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
引用次数: 9
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Abstract
Cytokine-producing cells are at the center of the adaptive immune responses, and quantifying these cells is an important aspect to build understanding of the immune response. In particular, Th1 and Th17 cells have been implicated in the pathogenesis of such diseases as inflammatory bowel disease, rheumatoid arthritis, and multiple sclerosis. Quantification of Th1 and Th17 cells can provide important information in research of these diseases and other Th1- and Th17-mediated immune disorders. In vitro stimulation of cells followed by surface and intracellular staining, presented here, has the advantage of detecting the cytokines directly instead of relying exclusively on surrogate surface markers which, although showing enrichment for the effector T cells, are not specific markers for the cytokine-producing cells. © 2015 by John Wiley & Sons, Inc.
PMA/离子霉素刺激后Th1和Th17细胞胞内染色的定量分析
细胞因子产生细胞是适应性免疫反应的中心,对这些细胞进行量化是了解免疫反应的一个重要方面。特别是,Th1和Th17细胞与炎症性肠病、类风湿性关节炎和多发性硬化症等疾病的发病机制有关。Th1和Th17细胞的定量可以为这些疾病以及其他Th1和Th17介导的免疫疾病的研究提供重要信息。在体外刺激细胞,然后进行表面和细胞内染色,具有直接检测细胞因子的优点,而不是完全依赖于替代表面标记物,尽管对效应T细胞显示富集,但不是细胞因子产生细胞的特异性标记物。©2015 by John Wiley &儿子,Inc。
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