A fungal sarcolemmal membrane-associated protein (SLMAP) homolog plays a fundamental role in development and localizes to the nuclear envelope, endoplasmic reticulum, and mitochondria.

Eukaryotic Cell Pub Date : 2015-04-01 Epub Date: 2014-12-19 DOI:10.1128/EC.00241-14
Steffen Nordzieke, Thomas Zobel, Benjamin Fränzel, Dirk A Wolters, Ulrich Kück, Ines Teichert
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引用次数: 50

Abstract

Sarcolemmal membrane-associated protein (SLMAP) is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. Further, SLMAP misexpression is associated with endothelial dysfunctions in diabetes and cancer. SLMAP is part of the conserved striatin-interacting phosphatase and kinase (STRIPAK) complex required for specific signaling pathways in yeasts, filamentous fungi, insects, and mammals. In filamentous fungi, STRIPAK was initially discovered in Sordaria macrospora, a model system for fungal differentiation. Here, we functionally characterize the STRIPAK subunit PRO45, a homolog of human SLMAP. We show that PRO45 is required for sexual propagation and cell-to-cell fusion and that its forkhead-associated (FHA) domain is essential for these processes. Protein-protein interaction studies revealed that PRO45 binds to STRIPAK subunits PRO11 and SmMOB3, which are also required for sexual propagation. Superresolution structured-illumination microscopy (SIM) further established that PRO45 localizes to the nuclear envelope, endoplasmic reticulum, and mitochondria. SIM also showed that localization to the nuclear envelope requires STRIPAK subunits PRO11 and PRO22, whereas for mitochondria it does not. Taken together, our study provides important insights into fundamental roles of the fungal SLMAP homolog PRO45 and suggests STRIPAK-related and STRIPAK-unrelated functions.

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真菌肌层膜相关蛋白(SLMAP)同源物在发育过程中起着重要作用,定位于核膜、内质网和线粒体。
肌上皮膜相关蛋白(SLMAP)是一种尾锚定蛋白,参与基本细胞过程,如成肌细胞融合、细胞周期进展和染色体遗传。此外,SLMAP错误表达与糖尿病和癌症的内皮功能障碍有关。SLMAP是保守的纹状蛋白相互作用磷酸酶和激酶(STRIPAK)复合体的一部分,该复合体在酵母、丝状真菌、昆虫和哺乳动物的特定信号通路中是必需的。在丝状真菌中,STRIPAK最初是在大孢子Sordaria macrospora中发现的,这是真菌分化的模式系统。在这里,我们对STRIPAK亚基PRO45进行了功能表征,PRO45是人类SLMAP的同源物。我们发现PRO45是有性繁殖和细胞间融合所必需的,并且它的叉头相关(FHA)结构域对这些过程至关重要。蛋白-蛋白相互作用研究表明,PRO45结合STRIPAK亚基PRO11和SmMOB3,这两个亚基也是有性繁殖所必需的。超分辨率结构照明显微镜(SIM)进一步证实PRO45定位于核膜、内质网和线粒体。SIM还显示,核膜的定位需要STRIPAK亚基PRO11和PRO22,而线粒体则不需要。总之,我们的研究为真菌SLMAP同源物PRO45的基本作用提供了重要的见解,并提出了与stripak相关和与stripak无关的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
自引率
0.00%
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0
审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
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