Investigation of B-Z transitions with DNA oligonucleotides containing 8-methylguanine.

Frederic Y-H Chen, Soyoung Park, Haruka Otomo, Sohei Sakashita, Hiroshi Sugiyama
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引用次数: 15

Abstract

Among various Z-form DNA inducers, such as transition metal complexes, polyamines and high ionic concentrations, 8-methylguanine have received attention as efficient chemical modifications. Although it is clear that m8-modified guanine base markedly stabilizes the Z conformation of short oligonucleotides under physiological salt conditions, how sequence composition affects the preference of Z-DNA is still not well established. In this study, various oligomers of d(CG)n or d(GC)n containing either 8-methylguanine in a different position were synthesized and their capacity of stabilizing Z-DNA were evaluated by CD spectra and then compared with each other. It is was found out that the Z-DNA stabilizing effect depend on the order of arrangement of m(8)G and m(8)rG in DNA strands and the center position is the most effective to stabilize the Z-DNA and promote the B to Z transition.

Abstract Image

Abstract Image

含8-甲基鸟嘌呤DNA寡核苷酸的B-Z跃迁研究。
在各种z型DNA诱导剂中,如过渡金属配合物、多胺和高离子浓度,8-甲基鸟嘌呤作为一种高效的化学修饰受到了人们的关注。虽然m8修饰的鸟嘌呤碱基在生理盐条件下明显稳定短寡核苷酸的Z构象,但序列组成如何影响Z- dna的偏好仍然没有很好的确定。本研究合成了不同位置含有8-甲基鸟嘌呤的d(CG)n或d(GC)n低聚物,用CD谱评价了它们稳定Z-DNA的能力,并进行了比较。结果表明,稳定Z-DNA的效果与m(8)G和m(8)rG在DNA链中的排列顺序有关,中心位置对稳定Z-DNA和促进B向Z过渡最有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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