Prior regular exercise reverses the decreased effects of sleep deprivation on brain-derived neurotrophic factor levels in the hippocampus of ovariectomized female rats

Regulatory Peptides Pub Date : 2014-11-01 DOI:10.1016/j.regpep.2014.11.004

Hakimeh Saadati , Vahid Sheibani , Saeed Esmaeili-Mahani , Fatemeh Darvishzadeh-Mahani , Shahrzad Mazhari

{"title":"Prior regular exercise reverses the decreased effects of sleep deprivation on brain-derived neurotrophic factor levels in the hippocampus of ovariectomized female rats","authors":"Hakimeh Saadati , Vahid Sheibani , Saeed Esmaeili-Mahani , Fatemeh Darvishzadeh-Mahani , Shahrzad Mazhari","doi":"10.1016/j.regpep.2014.11.004","DOIUrl":null,"url":null,"abstract":"<div>Previous studies indicated that brain-derived neurotrophic factor (BDNF) is the main candidate to mediate the beneficial effects of exercise on cognitive function in sleep deprived male rats. In addition, our previous findings demonstrate that female rats are more vulnerable to the deleterious effects of sleep deprivation on cognitive performance and synaptic plasticity.Therefore, the current study was designed to investigate the effects of treadmill exercise and/or sleep deprivation (SD) on the levels of BDNF mRNA and protein in the hippocampus of female rats.Intact and ovariectomized (OVX) female Wistar rats were used in the present experiment. The exercise protocol was four weeks treadmill running and sleep deprivation was accomplished using the multiple platform method. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analysis were used to evaluate the level of BDNF mRNA and protein in the rat hippocampus respectively.Our results showed that protein and mRNA expression of BDNF was significantly (p<0.05) decreased after 72h SD in OVX rats in compared with other groups. Furthermore, sleep deprived OVX rats under exercise conditions had a significant (p<0.05) up-regulation of the BDNF protein and mRNA in the hippocampus.These findings suggest that regular exercise can exert a protective effect against hippocampus-related functions and impairments induced by sleep deprivation probably by inducing BDNF expression.</div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"194 ","pages":"Pages 11-15"},"PeriodicalIF":0.0000,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.11.004","citationCount":"36","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regulatory Peptides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167011514000871","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 36

Abstract

Previous studies indicated that brain-derived neurotrophic factor (BDNF) is the main candidate to mediate the beneficial effects of exercise on cognitive function in sleep deprived male rats. In addition, our previous findings demonstrate that female rats are more vulnerable to the deleterious effects of sleep deprivation on cognitive performance and synaptic plasticity.

Therefore, the current study was designed to investigate the effects of treadmill exercise and/or sleep deprivation (SD) on the levels of BDNF mRNA and protein in the hippocampus of female rats.

Intact and ovariectomized (OVX) female Wistar rats were used in the present experiment. The exercise protocol was four weeks treadmill running and sleep deprivation was accomplished using the multiple platform method. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analysis were used to evaluate the level of BDNF mRNA and protein in the rat hippocampus respectively.

Our results showed that protein and mRNA expression of BDNF was significantly (p < 0.05) decreased after 72 h SD in OVX rats in compared with other groups. Furthermore, sleep deprived OVX rats under exercise conditions had a significant (p < 0.05) up-regulation of the BDNF protein and mRNA in the hippocampus.

These findings suggest that regular exercise can exert a protective effect against hippocampus-related functions and impairments induced by sleep deprivation probably by inducing BDNF expression.

查看原文本刊更多论文

先前有规律的运动逆转了睡眠剥夺对去卵巢雌性大鼠海马体中脑源性神经营养因子水平下降的影响

先前的研究表明，脑源性神经营养因子(BDNF)是介导运动对睡眠剥夺雄性大鼠认知功能有益影响的主要候选因子。此外，我们之前的研究结果表明，雌性大鼠更容易受到睡眠剥夺对认知表现和突触可塑性的有害影响。因此，本研究旨在探讨跑步机运动和/或睡眠剥夺(SD)对雌性大鼠海马BDNF mRNA和蛋白水平的影响。本实验采用完整和去卵巢雌性Wistar大鼠。运动方案为四周的跑步机跑步，并使用多平台方法完成睡眠剥夺。采用定量逆转录聚合酶链反应(RT-PCR)和免疫印迹法分别检测大鼠海马组织中BDNF mRNA和蛋白的表达水平。我们的结果显示，BDNF蛋白和mRNA的表达显著(p <OVX大鼠在72 h SD后与其他组相比明显降低(0.05)。此外，在运动条件下，睡眠剥夺的OVX大鼠具有显著的(p <0.05)海马BDNF蛋白和mRNA上调。这些发现表明，有规律的运动可能通过诱导BDNF表达，对睡眠剥夺引起的海马体相关功能和损伤起到保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.