nspA gene as a specific genetic marker for detection of Neisseria meningitidis causing bacterial meningitis.

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian journal of biochemistry & biophysics Pub Date : 2014-06-01

Neha Bhatt, Nazneen Khan, Sandip K Dash, Shashi Khare, Ashok Kumar

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引用次数: 0

Abstract

Bacterial meningitis caused by Neisseria meningitidis which causes human brain meninges damage, is generally diagnosed from patient cerebrospinal fluid through microscopy, immunological assays, biochemical test, PCR, microarray and biosensors. However, these methods are expensive, time-consuming or non-confirmatory due to certain limitations. A quick PCR based method was developed for detection of bacterial meningitis caused by N. meningitidis using specific primers based on amplification of virulence nspA (Neisseria surface protein A) gene partial sequence (202 bp). The nspA gene amplicon could be used as a genetic marker for minimum detection of 10 ng genomic DNA (G-DNA) of N. meningitidis with high sensitivity only in 80 min, which is least time reported for the confirmation of the disease. However, the lower detection limit was found as low as 1.0 ng G-DNA, but with less sensitivity. The cross-reactivity of the genetic marker, was also studied with other possible pathogens. A comparison with the presently available detection methods and our method was also done using patient samples.

本刊更多论文

nspA基因作为检测引起细菌性脑膜炎的脑膜炎奈瑟菌的特异性遗传标记。

细菌性脑膜炎由脑膜炎奈瑟菌引起，可导致人脑膜损伤，通常通过显微镜、免疫测定、生化试验、PCR、微阵列和生物传感器从患者脑脊液中诊断。然而，由于某些限制，这些方法昂贵、耗时或不确定。采用特异性引物扩增奈瑟菌表面蛋白A (Neisseria surface protein A)毒力基因部分序列(202bp)，建立了一种快速PCR检测奈瑟菌所致细菌性脑膜炎的方法。nspA基因扩增子可作为脑膜炎奈索菌10 ng基因组DNA (G-DNA)最小检测的遗传标记，仅在80 min内具有高灵敏度，这是报道的疾病确诊时间最短的遗传标记。然而，检测下限低至1.0 ng G-DNA，但灵敏度较低。并对该遗传标记与其他可能的病原菌的交叉反应性进行了研究。并与现有的检测方法和本方法进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian journal of biochemistry & biophysics 生物-生化与分子生物学

CiteScore

2.90

自引率

50.00%

发文量

审稿时长

3 months

期刊介绍： Started in 1964, this journal publishes original research articles in the following areas: structure-function relationships of biomolecules; biomolecular recognition, protein-protein and protein-DNA interactions; gene-cloning, genetic engineering, genome analysis, gene targeting, gene expression, vectors, gene therapy; drug targeting, drug design; molecular basis of genetic diseases; conformational studies, computer simulation, novel DNA structures and their biological implications, protein folding; enzymes structure, catalytic mechanisms, regulation; membrane biochemistry, transport, ion channels, signal transduction, cell-cell communication, glycobiology; receptors, antigen-antibody binding, neurochemistry, ageing, apoptosis, cell cycle control; hormones, growth factors; oncogenes, host-virus interactions, viral assembly and structure; intermediary metabolism, molecular basis of disease processes, vitamins, coenzymes, carrier proteins, toxicology; plant and microbial biochemistry; surface forces, micelles and microemulsions, colloids, electrical phenomena, etc. in biological systems. Solicited peer reviewed articles on contemporary Themes and Methods in Biochemistry and Biophysics form an important feature of IJBB. Review articles on a current topic in the above fields are also considered. They must dwell more on research work done during the last couple of years in the field and authors should integrate their own work with that of others with acumen and authenticity, mere compilation of references by a third party is discouraged. While IJBB strongly promotes innovative novel research works for publication as full length papers, it also considers research data emanating from limited objectives, and extension of ongoing experimental works as ‘Notes’. IJBB follows “Double Blind Review process” where author names, affiliations and other correspondence details are removed to ensure fare evaluation. At the same time, reviewer names are not disclosed to authors.