Hernan P Fainberg, Kayleigh L Almond, Dongfang Li, Cyril Rauch, Paul Bikker, Michael E Symonds, Alison Mostyn
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引用次数: 12
Abstract
Background: Maternal diet during pregnancy can modulate skeletal muscle development of the offspring. Previous studies in pigs have indicated that a fat supplemented diet during pregnancy can improve piglet outcome, however, this is in contrast to human studies suggesting adverse effects of saturated fats during pregnancy. This study aimed to investigate the impact of a fat supplemented (palm oil) "high fat" diet on skeletal muscle development in a porcine model. Histological and metabolic features of the biceps femoris muscle obtained from 7-day-old piglets born to sows assigned to either a commercial (C, n = 7) or to an isocaloric fat supplementation diet ("high fat" HF, n = 7) during pregnancy were assessed.
Results: Offspring exposed to a maternal HF diet demonstrated enhanced muscular development, reflected by an increase in fractional growth rate, rise in myofibre cross-sectional area, increased storage of glycogen and reduction in lipid staining of myofibres. Although both groups had similar intramuscular protein and triglyceride concentrations, the offspring born to HF mothers had a higher proportion of arachidonic acid (C20:4n6) and a reduction in α-linolenic acid (C18:3n3) compared to C group offspring. The HF group muscle also exhibited a higher ratio of C20:3n6 to C20:4n6 and total n-6 to n-3 in conjunction with up-regulation of genes associated with free fatty acid uptake and biogenesis.
Conclusion: In conclusion, a HF gestational diet accelerates the maturation of offspring biceps femoris muscle, reflected in increased glycolytic metabolism and fibre cross sectional area, differences accompanied with a potential resetting of myofibre nutrient uptake.
背景:孕期母体饮食可调节子代骨骼肌发育。先前对猪的研究表明,怀孕期间的脂肪补充饲料可以改善仔猪的结局,然而,这与人类研究表明的怀孕期间饱和脂肪的不利影响形成鲜明对比。这项研究旨在调查一种脂肪补充剂(棕榈油)的影响“高脂肪”饮食对猪骨骼肌发育的影响在妊娠期间,母猪分别饲喂商业饲粮(C, n = 7)和等热量脂肪补充饲粮(“高脂肪”HF, n = 7),对仔猪所生的7日龄仔猪的股二头肌的组织学和代谢特征进行了评估。结果:暴露于母体HF饮食的后代表现出增强的肌肉发育,反映在分数生长速率的增加,肌纤维横截面积的增加,糖原储存的增加和肌纤维脂质染色的减少。虽然两组的肌内蛋白和甘油三酯浓度相似,但与C组的后代相比,HF母亲所生的后代花生四烯酸(C20:4n6)的比例更高,α-亚麻酸(C18:3n3)的比例更低。HF组肌肉中C20:3n6比C20:4n6和总n-6比n-3的比例也更高,这与游离脂肪酸摄取和生物发生相关基因的上调有关。结论:总之,HF妊娠日粮加速了子代股二头肌的成熟,表现为糖酵解代谢和纤维横截面积的增加,这种差异伴随着肌纤维营养摄取的潜在重置。
BMC PhysiologyBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
9.60
自引率
0.00%
发文量
0
期刊介绍:
BMC Physiology is an open access journal publishing original peer-reviewed research articles in cellular, tissue-level, organismal, functional, and developmental aspects of physiological processes. BMC Physiology (ISSN 1472-6793) is indexed/tracked/covered by PubMed, MEDLINE, BIOSIS, CAS, EMBASE, Scopus, Zoological Record and Google Scholar.