The effect of shiga toxin on weibel-palade bodies in primary human endothelial cells.

Nephron Extra Pub Date : 2014-07-08 eCollection Date: 2014-01-01 DOI:10.1159/000363299

Joyce Geelen, Maartje van den Biggelaar, Peter Linssen, Thea van der Velden, Koen Mertens, Leo Monnens

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引用次数: 1

Abstract

Background/aims: Diarrhea-associated hemolytic uremic syndrome is associated with the presence of Shiga toxin (Stx1, Stx2 and several variants) in the circulation. The aim of this study is to examine the possible triggering effect of Stx1 on the exocytosis of Weibel-Palade bodies (WPbs).

Methods: Cultured human umbilical venous endothelial cells (HUVECs) and glomerular microvascular endothelial cells (GMVECs) were stimulated by thrombin and Stx1 in both static and flowing conditions. The amount of secreted von Willebrand factor (VWF) in the supernatant as well as the remaining intracellular fraction was determined.

Results: In HUVECs and in 2 out of 4 GMVECs, the stimulation of Stx1 in flow at 1 dyne/cm(2) resulted in a decrease of intracellular VWF. This is contrary to the results of Stx1 applied in static conditions. At a higher flow rate of 5 dyne/cm(2), no effect in GMVECs was observed.

Conclusion: Stx1 can contribute, via an effect on WPbs, to the exocytosis of WPbs in flow conditions in HUVECs and probably in GMVECs. This results in the release of VWF, suggesting an initiating role of the coagulation system in the pathogenesis.

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志贺毒素对人原代内皮细胞韦贝尔-腭体的影响。

背景/目的:腹泻相关溶血性尿毒症综合征与血液循环中志贺毒素(Stx1, Stx2和几种变体)的存在有关。本研究的目的是探讨Stx1对韦贝尔-帕拉德体(WPbs)胞吐的可能触发作用。方法:采用凝血酶和Stx1对培养的人脐静脉内皮细胞(HUVECs)和肾小球微血管内皮细胞(GMVECs)进行静态和流动刺激。测定上清液中血管性血友病因子(VWF)的分泌量以及剩余的细胞内分数。结果:在huvec和2 / 4的gmvec中，以1 dyne/cm(2)的流量刺激Stx1导致细胞内VWF降低。这与Stx1在静态条件下应用的结果相反。在更高的流量为5达因/厘米(2)时，对gmvec没有影响。结论:Stx1可以通过对WPbs的影响，促进huvec和gmvec在流动条件下的WPbs的分泌。这导致VWF的释放，提示凝血系统在发病机制中的启动作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nephron Extra

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： An open-access subjournal to Nephron. ''Nephron EXTRA'' publishes additional high-quality articles that cannot be published in the main journal ''Nephron'' due to space limitations.